PMID- 1990295
OWN - NLM
STAT- MEDLINE
DCOM- 19910304
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 11
IP  - 2
DP  - 1991 Feb
TI  - Control of junB and extracellular matrix protein expression by transforming 
      growth factor-beta 1 is independent of simian virus 40 T antigen-sensitive 
      growth-sensitive growth-inhibitory events.
PG  - 972-8
AB  - Treatment of Mv1Lu mink lung epithelial cells with transforming growth 
      factor-beta 1 (TGF-beta 1) prevents phosphorylation of the retinoblastoma 
      susceptibility gene product, RB, in late G1 phase of the cell cycle, which is 
      thought to retain RB in a growth-suppressive state. This effect is paralleled by 
      cell cycle arrest in late G1 (M. Laiho, J. A. DeCapric, J. W. Ludlow, D. M. 
      Livingston, and J. Massague, Cell 62:175-185, 1990). Arrest can be prevented by 
      expression of simian virus 40 T antigen, which binds to underphosphorylated RB, 
      presumably blocking its growth-suppressive activity. The response of cells to 
      TGF-beta 1, however, is complex and includes changes in the levels of expression 
      of genes encoding nuclear transcription factors and extracellular matrix 
      components. To define the relationships among various components of the TGF-beta 
      1 response, we have investigated the effect of TGF-beta 1 on cells whose 
      growth-inhibitory response to this factor is prevented by T antigen. TGF-beta 1 
      addition to exponentially growing Mv1Lu cells increased the levels of junB mRNA 
      and of three extracellular matrix proteins: plasminogen activator inhibitor-1, 
      fibronectin, and thrombospondin. Kinetically, the effects on junB and plasminogen 
      activator inhibitor-1 expression occurred faster (half-maximal at 1 to 2 h) than 
      the effects on fibronectin and thrombospondin expression (half-maximal at 6 to 10 
      h). These effects either preceded or overlapped, respectively, the withdrawal of 
      Mv1Lu cells from the cell cycle. Expression of a transfected T-antigen gene in 
      Mv1Lu cells, however, did not prevent any of these responses to TGF-beta 1. The 
      results indcate that TGF-B1-stimulated expression of junB and extracellular 
      matrix proteins in Mv1Lu cells can occur independently of the T-antigen-sensitive 
      events that lead to growth arrest.
FAU - Laiho, M
AU  - Laiho M
AD  - Howard Hughes Medical Institute, Memorial Sloan-Kettering Cancer Center, New 
      York, New York 10021.
FAU - Ronnstrand, L
AU  - Ronnstrand L
FAU - Heino, J
AU  - Heino J
FAU - Decaprio, J A
AU  - Decaprio JA
FAU - Ludlow, J W
AU  - Ludlow JW
FAU - Livingston, D M
AU  - Livingston DM
FAU - Massague, J
AU  - Massague J
LA  - eng
GR  - CA 34610/CA/NCI NIH HHS/United States
GR  - CA 39240/CA/NCI NIH HHS/United States
GR  - CA 50661/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Fibronectins)
RN  - 0 (Plasminogen Inactivators)
RN  - 0 (Platelet Membrane Glycoproteins)
RN  - 0 (Proto-Oncogene Proteins c-jun)
RN  - 0 (RNA, Messenger)
RN  - 0 (Thrombospondins)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Cell Cycle
MH  - Cell Line
MH  - DNA-Binding Proteins/*genetics
MH  - Extracellular Matrix/*metabolism
MH  - Fibronectins/*genetics
MH  - Gene Expression Regulation
MH  - Genes, Retinoblastoma/drug effects
MH  - Lung
MH  - Mink
MH  - Plasminogen Inactivators/*metabolism
MH  - Platelet Membrane Glycoproteins/*genetics
MH  - Proto-Oncogene Proteins c-jun
MH  - RNA, Messenger/genetics/isolation & purification
MH  - Thrombospondins
MH  - Transfection
MH  - Transforming Growth Factor beta/*pharmacology
PMC - PMC359761
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
PMCR- 1991/02/01
CRDT- 1991/02/01 00:00
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
PHST- 1991/02/01 00:00 [entrez]
PHST- 1991/02/01 00:00 [pmc-release]
AID - 10.1128/mcb.11.2.972-978.1991 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1991 Feb;11(2):972-8. doi: 10.1128/mcb.11.2.972-978.1991.