PMID- 19903100
OWN - NLM
STAT- MEDLINE
DCOM- 20100603
LR  - 20220311
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 11
IP  - 7
DP  - 2009
TI  - Properties and growth of human bone marrow mesenchymal stromal cells cultivated 
      in different media.
PG  - 874-85
LID - 10.3109/14653240903188947 [doi]
AB  - BACKGROUND AIMS: Human mesenchymal stromal cells (hMSC) are a promising tool for 
      future clinical application, but their use requires rapid cell expansion in media 
      suitable for clinical use. Therefore, we tested the influence of several culture 
      media on colony formation, population doubling (PD) time, cell cycle and surface 
      marker expression. METHODS: hMSC isolated from human bone marrow (BM) obtained 
      from healthy donors were seeded and expanded in different culture media: 
      alpha-minimum essential medium (MEM) supplemented with 2.5%, 5%, 10% or 20% fetal 
      bovine serum (FBS), 5% or 10% human cord blood serum (hCBS), 5% or 10% human 
      blood serum from AB adult donors (hABS), or mesenchymal stem cell growth medium 
      (MSCGM). The number, diameter and total area of the colonies formed and PD time 
      were determined, and the cell cycle and 16 surface markers were analyzed. 
      RESULTS: Colony-forming efficiency was best in alpha-MEM/hCBS and alpha-MEM/hABS, 
      good in MSCGM and worst in alpha-MEM/FBS. The shortest PD time was achieved in 
      media enriched with human sera or MSCGM, while the time was increased in 
      alpha-MEM/FBS. The largest proliferating fraction was seen in MSCGM followed by 
      media enriched with human sera; the fraction was smallest in alpha-MEM/FBS. 
      Staining for CD34, CD45, CD235a and CD271 was negative, while that for CD29, 
      CD44, CD73, CD90, CD105 and human leukocyte antigen (HLA)-A, -B, -C was positive 
      in all media tested. Media with human serum did not adversely affect the 
      differentiation potential of hMSC, and differentiation into osteoblasts was 
      enhanced. CONCLUSIONS: The choice of serum influences hMSC expansion and cell 
      properties; alpha-MEM supplemented with hABS seems to be a promising candidate 
      for clinical use.
FAU - Turnovcova, Karolina
AU  - Turnovcova K
AD  - Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, 
      Prague, Czech Republic.
FAU - Ruzickova, Katerina
AU  - Ruzickova K
FAU - Vanecek, Vaclav
AU  - Vanecek V
FAU - Sykova, Eva
AU  - Sykova E
FAU - Jendelova, Pavla
AU  - Jendelova P
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (Culture Media, Conditioned)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - Antigens, Differentiation/metabolism
MH  - Bone Marrow Cells/cytology/*metabolism
MH  - Cell Culture Techniques
MH  - Cell Differentiation
MH  - Cell Growth Processes
MH  - Colony-Forming Units Assay
MH  - Culture Media, Conditioned/*metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - Stromal Cells/cytology/*metabolism
EDAT- 2009/11/12 06:00
MHDA- 2010/06/04 06:00
CRDT- 2009/11/12 06:00
PHST- 2009/11/12 06:00 [entrez]
PHST- 2009/11/12 06:00 [pubmed]
PHST- 2010/06/04 06:00 [medline]
AID - 10.3109/14653240903188947 [pii]
AID - 10.3109/14653240903188947 [doi]
PST - ppublish
SO  - Cytotherapy. 2009;11(7):874-85. doi: 10.3109/14653240903188947.