PMID- 19907862
OWN - NLM
STAT- MEDLINE
DCOM- 20150619
LR  - 20181201
IS  - 1006-7248 (Print)
IS  - 1006-7248 (Linking)
VI  - 18
IP  - 5
DP  - 2009 Oct
TI  - [Effects of ADAM28 AS-ODN on proliferation, differentiation and apoptosis of 
      HPDLSC].
PG  - 524-31
AB  - PURPOSE: To investigate the effects of a disintegrin and metalloproteinase 
      28(ADAM28) antisense oligodeoxynucleotide (AS-ODN) on proliferation, 
      differentiation and apoptosis of human periodontal ligament stem cell(HPDLSC) and 
      possible mechanism. METHODS: HPDLSC was isolated, cultured in vitro and 
      identified. Synthetic ADAM28 AS-ODN and S-ODN were transfected into HPDLSC, 
      respectively. MTT chromatometry, enzyme dynamics and flow cytometry (FCM) were 
      used to detect the effects of ADAM28 AS-ODN on biological characteristics of 
      HPDLSC. Statistical significance was assessed by the Student-Newman-Keuls (SNK) 
      test with SPSS 13.0 software package. RESULTS: In ADAM28 AS-ODN group, the 
      proliferation activity and index of HPDLSC were significantly lower than those of 
      S-ODN group and untransfected group, alkaline phosphatase(ALP) secretion level 
      and percentage of apoptotic cells went up obviously. Significant difference was 
      detected between AS-ODN group and other groups (P<0.01). CONCLUSIONS: ADAM28 
      AS-ODN could inhibit HPDLSC proliferation and influence the changes of cell 
      cycle, promote ALP secretion and induce HPDLSC apoptosis significantly.
FAU - Zhao, Zheng
AU  - Zhao Z
AD  - Institute of Stomatology, General Hospital of Chinese PLA, Beijing, China. 
      zhaozheng170@sohu.com
FAU - Liu, Hong-chen
AU  - Liu HC
FAU - Huang, Zheng-nan
AU  - Huang ZN
LA  - chi
PT  - Journal Article
PL  - China
TA  - Shanghai Kou Qiang Yi Xue
JT  - Shanghai kou qiang yi xue = Shanghai journal of stomatology
JID - 101090220
RN  - 0 (Oligonucleotides, Antisense)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - EC 3.4.24.- (ADAM Proteins)
RN  - EC 3.4.24.- (ADAM28 protein, human)
SB  - IM
MH  - *ADAM Proteins
MH  - Alkaline Phosphatase
MH  - Apoptosis
MH  - Cell Cycle
MH  - *Cell Differentiation
MH  - *Cell Proliferation
MH  - Humans
MH  - *Oligonucleotides, Antisense
MH  - Periodontal Ligament
MH  - *Stem Cells
MH  - Transfection
EDAT- 2009/11/13 06:00
MHDA- 2015/06/20 06:00
CRDT- 2009/11/13 06:00
PHST- 2009/11/13 06:00 [entrez]
PHST- 2009/11/13 06:00 [pubmed]
PHST- 2015/06/20 06:00 [medline]
PST - ppublish
SO  - Shanghai Kou Qiang Yi Xue. 2009 Oct;18(5):524-31.