PMID- 19909545
OWN - NLM
STAT- MEDLINE
DCOM- 20100126
LR  - 20211020
IS  - 1746-160X (Electronic)
IS  - 1746-160X (Linking)
VI  - 5
DP  - 2009 Nov 12
TI  - In vitro proliferation of human osteogenic cells in presence of different 
      commercial bone substitute materials combined with enamel matrix derivatives.
PG  - 23
LID - 10.1186/1746-160X-5-23 [doi]
AB  - BACKGROUND: Cellular reactions to alloplastic bone substitute materials (BSM) are 
      a subject of interest in basic research. In regenerative dentistry, these bone 
      grafting materials are routinely combined with enamel matrix derivatives (EMD) in 
      order to additionally enhance tissue regeneration. MATERIALS AND METHODS: The aim 
      of this study was to evaluate the proliferative activity of human osteogenic 
      cells after incubation over a period of seven days with commercial BSM of various 
      origin and chemical composition. Special focus was placed on the potential 
      additional benefit of EMD on cellular proliferation. RESULTS: Except for 
      PerioGlas, osteogenic cell proliferation was significantly promoted by the 
      investigated BSM. The application of EMD alone also resulted in significantly 
      increased cellular proliferation. However, a combination of BSM and EMD resulted 
      in only a moderate additional enhancement of osteogenic cell proliferation. 
      CONCLUSION: The application of most BSM, as well as the exclusive application of 
      EMD demonstrated a positive impact on the proliferation of human osteogenic cells 
      in vitro. In order to increase the benefit from substrate combination (BSM + 
      EMD), further studies on the interactions between BSM and EMD are needed.
FAU - Reichert, Christoph
AU  - Reichert C
AD  - Department of Oral and Maxillofacial Surgery, University Hospital Aachen, Aachen, 
      Germany. c_reichert@web.de
FAU - Al-Nawas, Bilal
AU  - Al-Nawas B
FAU - Smeets, Ralf
AU  - Smeets R
FAU - Kasaj, Adrian
AU  - Kasaj A
FAU - Gotz, Werner
AU  - Gotz W
FAU - Klein, Marcus O
AU  - Klein MO
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091112
PL  - England
TA  - Head Face Med
JT  - Head & face medicine
JID - 101245792
RN  - 0 (Bone Substitutes)
RN  - 0 (Dental Enamel Proteins)
RN  - 0 (Indicators and Reagents)
RN  - 0 (Oxazines)
RN  - 0 (Xanthenes)
RN  - 0 (enamel matrix proteins)
RN  - 1FN9YD6968 (resazurin)
SB  - IM
MH  - Bone Regeneration/*physiology
MH  - Bone Substitutes/*pharmacology
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Dental Enamel Proteins/*pharmacology
MH  - Humans
MH  - In Vitro Techniques
MH  - Indicators and Reagents
MH  - Osteoblasts/*physiology
MH  - Osteogenesis/*physiology
MH  - Oxazines
MH  - Xanthenes
PMC - PMC2780983
EDAT- 2009/11/17 06:00
MHDA- 2010/01/27 06:00
PMCR- 2009/11/12
CRDT- 2009/11/14 06:00
PHST- 2009/05/23 00:00 [received]
PHST- 2009/11/12 00:00 [accepted]
PHST- 2009/11/14 06:00 [entrez]
PHST- 2009/11/17 06:00 [pubmed]
PHST- 2010/01/27 06:00 [medline]
PHST- 2009/11/12 00:00 [pmc-release]
AID - 1746-160X-5-23 [pii]
AID - 10.1186/1746-160X-5-23 [doi]
PST - epublish
SO  - Head Face Med. 2009 Nov 12;5:23. doi: 10.1186/1746-160X-5-23.