PMID- 19910049
OWN - NLM
STAT- MEDLINE
DCOM- 20100219
LR  - 20211020
IS  - 1872-9142 (Electronic)
IS  - 0161-5890 (Print)
IS  - 0161-5890 (Linking)
VI  - 47
IP  - 4
DP  - 2010 Jan
TI  - Reoxygenation of hypoxia-differentiated dentritic cells induces Th1 and Th17 cell 
      differentiation.
PG  - 922-31
LID - 10.1016/j.molimm.2009.09.038 [doi]
AB  - Dendritic cells (DCs) are often exposed to various oxygen tensions under 
      physiological and pathological conditions. However, the effects of various oxygen 
      tensions on DC functions remain unclear. In this study, we showed that 
      hypoxia-differentiated DCs expressed lower levels of MHC-II molecule, 
      co-stimulatory molecules (CD80, CD86) and proinflammatory cytokines (IL-1beta, 
      IL-6, and TNF-alpha), but higher levels of immunoregulatory cytokine transforming 
      growth factor-beta (TGF-beta) than normoxia-differentiated DCs. Unexpectedly, 
      re-exposure of hypoxia-differentiated DCs to saturated oxygen (reoxygenation) 
      completely restored their mature phenotype and function. Specifically, the 
      reoxygenated DCs induced naive CD4(+) T cells to differentiate into Th1 and Th17 
      effector cells, but deceased the generation of CD4(+)CD25(+)Foxp3(+) regulatory T 
      cells (Tregs). The data indicate that hypoxic microenvironment suppresses the 
      maturation and function of murine DCs. Reoxygenation of hypoxia-differentiated 
      DCs however results in complete recovery of their mature phenotype and function, 
      and has strong ability to drive immune response toward a proinflammatory 
      direction, suggesting reoxygenated DCs may contribute to inflammation of 
      ischemia-reperfusion injury.
CI  - Published by Elsevier Ltd.
FAU - Wang, Qun
AU  - Wang Q
AD  - Department of Immunology, Shandong University School of Medicine, 44# Wenhua Xi 
      Road, Jinan 250012, China.
FAU - Liu, Chunmei
AU  - Liu C
FAU - Zhu, Faliang
AU  - Zhu F
FAU - Liu, Fengming
AU  - Liu F
FAU - Zhang, Pin
AU  - Zhang P
FAU - Guo, Chun
AU  - Guo C
FAU - Wang, Xiaoyan
AU  - Wang X
FAU - Li, Haiyan
AU  - Li H
FAU - Ma, Chunhong
AU  - Ma C
FAU - Sun, Wensheng
AU  - Sun W
FAU - Zhang, Yun
AU  - Zhang Y
FAU - Chen, Wanjun
AU  - Chen W
FAU - Zhang, Lining
AU  - Zhang L
LA  - eng
GR  - ZIA DE000101-06/ImNIH/Intramural NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Intramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20091112
PL  - England
TA  - Mol Immunol
JT  - Molecular immunology
JID - 7905289
RN  - 0 (CD4 Antigens)
RN  - 0 (Cytokines)
RN  - 0 (Forkhead Transcription Factors)
RN  - 0 (Foxp3 protein, mouse)
RN  - 0 (Interleukin-2 Receptor alpha Subunit)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - CD4 Antigens/immunology
MH  - Cell Differentiation/*drug effects
MH  - Cell Hypoxia/drug effects
MH  - Cell Proliferation/drug effects
MH  - Coculture Techniques
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*cytology/*drug effects/immunology
MH  - Female
MH  - Forkhead Transcription Factors/immunology
MH  - Interleukin-2 Receptor alpha Subunit/immunology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Oxygen/*pharmacology
MH  - Phenotype
MH  - T-Lymphocytes, Helper-Inducer/*cytology/*drug effects/immunology
MH  - T-Lymphocytes, Regulatory/cytology/drug effects/immunology
MH  - Th1 Cells/cytology/drug effects/immunology
PMC - PMC2815172
MID - NIHMS158766
EDAT- 2009/11/17 06:00
MHDA- 2010/02/20 06:00
PMCR- 2011/01/01
CRDT- 2009/11/14 06:00
PHST- 2009/07/24 00:00 [received]
PHST- 2009/09/24 00:00 [revised]
PHST- 2009/09/30 00:00 [accepted]
PHST- 2009/11/14 06:00 [entrez]
PHST- 2009/11/17 06:00 [pubmed]
PHST- 2010/02/20 06:00 [medline]
PHST- 2011/01/01 00:00 [pmc-release]
AID - S0161-5890(09)00752-4 [pii]
AID - 10.1016/j.molimm.2009.09.038 [doi]
PST - ppublish
SO  - Mol Immunol. 2010 Jan;47(4):922-31. doi: 10.1016/j.molimm.2009.09.038. Epub 2009 
      Nov 12.