PMID- 19910630
OWN - NLM
STAT- MEDLINE
DCOM- 20100208
LR  - 20181201
IS  - 1526-7598 (Electronic)
IS  - 0003-2999 (Linking)
VI  - 110
IP  - 2
DP  - 2010 Feb 1
TI  - The effect of hydroxyethyl starches (HES 130/0.42 and HES 200/0.5) on activated 
      renal tubular epithelial cells.
PG  - 531-40
LID - 10.1213/ANE.0b013e3181c03c97 [doi]
AB  - BACKGROUND: Acute renal failure is a frequent complication of sepsis. 
      Hydroxyethyl starch (HES) is widely used in the treatment of such patients. 
      However, the effect of HES on renal function during sepsis remains controversial. 
      We established an in vitro model of tumor necrosis factor-alpha 
      (TNF-alpha)-stimulated human proximal tubular epithelial (HK-2) cells to assess 
      the possible effects of HES 130/0.42 and HES 200/0.5 on these activated cells. 
      METHODS: HK-2 cells were stimulated with TNF-alpha in the presence or absence of 
      HES 130/0.42 or 200/0.5. After 4, 10, and 18 h of incubation, monocyte 
      chemoattractant protein-1 (MCP-1), a key chemoattractant for neutrophils and 
      macrophages, was measured. In addition, viability and cytotoxicity assays were 
      performed. RESULTS: MCP-1 expression was doubled upon TNF-alpha exposure. In the 
      presence of 2% and 4% HES 200/0.5 in 98% (96%) medium over a stimulation time 
      period of 10 h and 18 h, the MCP-1 concentration was decreased between 26% and 
      56% (P < 0.05). TNF-alpha stimulation resulted in a significant decrease of 
      viability by 53%-63%, whereas viability decreased by only 32%-40% in coincubation 
      with HES 130/0.42 (P < 0.005) and remained even less affected by TNF-alpha in the 
      presence of HES 200/0.5 (P < 0.001). The TNF-alpha-induced cell death rate was 
      attenuated in the presence of HES 200/0.5 (P < 0.05). CONCLUSIONS: This in vitro 
      study shows that both HES products modulate cell injury upon inflammatory 
      stimulation. The effect was more pronounced in the HES 200/0.5 group than for HES 
      130/0.42, suggesting a possible biological difference between the HES types.
FAU - Wittlinger, Moritz
AU  - Wittlinger M
AD  - Institute of Anesthesiology, University of Zurich, Zurich, Switzerland.
FAU - Schlapfer, Martin
AU  - Schlapfer M
FAU - De Conno, Elisena
AU  - De Conno E
FAU - Z'graggen, Birgit Roth
AU  - Z'graggen BR
FAU - Reyes, Livia
AU  - Reyes L
FAU - Booy, Christa
AU  - Booy C
FAU - Schimmer, Ralph C
AU  - Schimmer RC
FAU - Seifert, Burkhardt
AU  - Seifert B
FAU - Burmeister, Marc-Alexander
AU  - Burmeister MA
FAU - Spahn, Donat R
AU  - Spahn DR
FAU - Beck-Schimmer, Beatrice
AU  - Beck-Schimmer B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091112
PL  - United States
TA  - Anesth Analg
JT  - Anesthesia and analgesia
JID - 1310650
RN  - 0 (Chemokine CCL2)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Hydroxyethyl Starch Derivatives)
RN  - 0 (Isotonic Solutions)
RN  - 0 (Plasma Substitutes)
RN  - 0 (Ringer's Lactate)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9000-70-8 (Gelatin)
RN  - 9049-67-6 (physiogel)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - I223NX31W9 (Fluorescein-5-isothiocyanate)
SB  - IM
CIN - Anesth Analg. 2010 Feb 1;110(2):300-1. PMID: 20081129
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Chemokine CCL2/biosynthesis
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Epithelial Cells/drug effects/metabolism
MH  - Fluorescein-5-isothiocyanate
MH  - Fluorescent Dyes
MH  - Gelatin/pharmacology
MH  - Humans
MH  - Hydroxyethyl Starch Derivatives/chemistry/*pharmacology
MH  - Inflammation
MH  - Isotonic Solutions/pharmacology
MH  - Kidney Tubules, Proximal/*drug effects/metabolism/pathology
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Microscopy, Confocal
MH  - Plasma Substitutes/*pharmacology
MH  - Ringer's Lactate
MH  - Tumor Necrosis Factor-alpha/pharmacology
EDAT- 2009/11/17 06:00
MHDA- 2010/02/09 06:00
CRDT- 2009/11/14 06:00
PHST- 2009/11/14 06:00 [entrez]
PHST- 2009/11/17 06:00 [pubmed]
PHST- 2010/02/09 06:00 [medline]
AID - ANE.0b013e3181c03c97 [pii]
AID - 10.1213/ANE.0b013e3181c03c97 [doi]
PST - ppublish
SO  - Anesth Analg. 2010 Feb 1;110(2):531-40. doi: 10.1213/ANE.0b013e3181c03c97. Epub 
      2009 Nov 12.