PMID- 19910691
OWN - NLM
STAT- MEDLINE
DCOM- 20100217
LR  - 20190212
IS  - 1421-9778 (Electronic)
IS  - 1015-8987 (Linking)
VI  - 24
IP  - 5-6
DP  - 2009
TI  - The role of chemokines in proangiogenic action induced by human adipose 
      tissue-derived mesenchymal stem cells in the murine model of hindlimb ischemia.
PG  - 511-8
LID - 10.1159/000257495 [doi]
AB  - The proangiogenic action of human adipose tissue-derived mesenchymal stem cells 
      (hASCs) transplantation has been shown to be mediated by secretory factors. In 
      this study, we determined if human granulocyte chemotactic protein-2(GCP2) or 
      monocyte chemoattractant protein-1(MCP1) is involved in the proangiogenic action 
      of hASCs transplantation in the hindlimb ischemia model. hASCs secrete GCP2 and 
      MCP1, which leads to increased tubule formation. The downregulation of GCP2 or 
      MCP1 decreased MCP1 and GCP2 secretion, respectively, whereas the external 
      addition of GCP2 or MCP1 increased MCP1 and GCP2, respectively. Additionally, the 
      treatment of GCP2 and MCP1 increased VEGF secretion, while the downregulation of 
      GCP2 and MCP1 showed the opposite effect on VEGF secretion. Downregulation of 
      GCP2 and MCP1 expression also inhibited hASCs-induced proangiogenic action, while 
      the overexpression of GCP2 increased it. Finally, the downregulation of MCP1 or 
      VEGF inhibited the GCP2 overexpression-induced increase in blood flow recovery. 
      Taken together, these data indicate that the proangiogenic action of hASCs 
      transplantation is mediated by the interaction between GCP2, MCP1 and VEGF, which 
      are secreted from the transplanted cells.
CI  - 2009 S. Karger AG, Basel.
FAU - Cho, Hyun Hwa
AU  - Cho HH
AD  - Department of Physiology, Pusan National University, Yangsan, Korea.
FAU - Kim, Yeon Jeong
AU  - Kim YJ
FAU - Kim, Jong Tae
AU  - Kim JT
FAU - Song, Ji Sun
AU  - Song JS
FAU - Shin, Keun Koo
AU  - Shin KK
FAU - Bae, Yong Chan
AU  - Bae YC
FAU - Jung, Jin Sup
AU  - Jung JS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091104
PL  - Germany
TA  - Cell Physiol Biochem
JT  - Cellular physiology and biochemistry : international journal of experimental 
      cellular physiology, biochemistry, and pharmacology
JID - 9113221
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CXCL6)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Animals
MH  - Chemokine CCL2/genetics/metabolism/*physiology
MH  - Chemokine CXCL6/genetics/metabolism/*physiology
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Female
MH  - Hindlimb
MH  - Humans
MH  - Ischemia/therapy
MH  - Male
MH  - *Mesenchymal Stem Cell Transplantation
MH  - Mice
MH  - Mice, Nude
MH  - Middle Aged
MH  - *Neovascularization, Physiologic
MH  - RNA, Small Interfering/metabolism
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
EDAT- 2009/11/17 06:00
MHDA- 2010/02/18 06:00
CRDT- 2009/11/14 06:00
PHST- 2009/09/16 00:00 [accepted]
PHST- 2009/11/14 06:00 [entrez]
PHST- 2009/11/17 06:00 [pubmed]
PHST- 2010/02/18 06:00 [medline]
AID - 000257495 [pii]
AID - 10.1159/000257495 [doi]
PST - ppublish
SO  - Cell Physiol Biochem. 2009;24(5-6):511-8. doi: 10.1159/000257495. Epub 2009 Nov 
      4.