PMID- 19912575
OWN - NLM
STAT- MEDLINE
DCOM- 20100618
LR  - 20100303
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Linking)
VI  - 75
IP  - 2
DP  - 2010 Feb
TI  - Higher HLA class I expression in renal cell carcinoma than in autologous normal 
      tissue.
PG  - 110-8
LID - 10.1111/j.1399-0039.2009.01409.x [doi]
AB  - A total of 93 frozen primary renal cell carcinoma (RCC) samples and 31 frozen 
      samples of corresponding normal renal tissue were analyzed for human leukocyte 
      antigen (HLA) class I and HLA-DR expression. Unexpectedly, HLA class I expression 
      was much higher on RCC cells than on normal renal tubular cells. 
      Immunohistochemistry analysis of frozen and paraffin-embedded tissue samples, 
      applying an extended panel of specific anti-HLA monoclonal antibodies, showed 
      elevated HLA class I antigen expression in 95.6% of the tumors vs only 12.9% of 
      normal renal tissues. These findings were confirmed by molecular analysis of HLA 
      heavy chain and beta2-microglobulin (beta2m) transcription levels using 
      quantitative real-time polymerase chain reaction (PCR) on microdissected tissue 
      samples (isolated tumor nests and autologous normal renal tubules) from four 
      patients. These results might help to explain the relatively high success rate of 
      immunotherapy in patients with RCC. The molecular mechanism underlying the 
      increased HLA class I expression in RCC has yet to be elucidated.
FAU - Saenz-Lopez, P
AU  - Saenz-Lopez P
AD  - Servicio de Analisis Clinicos, Hospital Universitario Virgen de las Nieves, 
      Granada, Spain.
FAU - Gouttefangeas, C
AU  - Gouttefangeas C
FAU - Hennenlotter, J
AU  - Hennenlotter J
FAU - Concha, A
AU  - Concha A
FAU - Maleno, I
AU  - Maleno I
FAU - Ruiz-Cabello, F
AU  - Ruiz-Cabello F
FAU - Cozar, J M
AU  - Cozar JM
FAU - Tallada, M
AU  - Tallada M
FAU - Stenzl, A
AU  - Stenzl A
FAU - Rammensee, H-G
AU  - Rammensee HG
FAU - Garrido, F
AU  - Garrido F
FAU - Cabrera, T
AU  - Cabrera T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091113
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Histocompatibility Antigens Class I)
SB  - IM
MH  - Carcinoma, Renal Cell/genetics/*immunology/*pathology
MH  - HLA-DR Antigens/biosynthesis/*genetics/immunology
MH  - Histocompatibility Antigens Class I/*analysis/genetics/immunology
MH  - Humans
MH  - Immunohistochemistry
MH  - Kidney/chemistry/immunology
MH  - Kidney Neoplasms/genetics/*immunology/*pathology
MH  - Leukocytes/chemistry/immunology/pathology
MH  - Paraffin Embedding
EDAT- 2009/11/17 06:00
MHDA- 2010/06/19 06:00
CRDT- 2009/11/17 06:00
PHST- 2009/11/17 06:00 [entrez]
PHST- 2009/11/17 06:00 [pubmed]
PHST- 2010/06/19 06:00 [medline]
AID - TAN1409 [pii]
AID - 10.1111/j.1399-0039.2009.01409.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2010 Feb;75(2):110-8. doi: 10.1111/j.1399-0039.2009.01409.x. 
      Epub 2009 Nov 13.