PMID- 19912778
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20191111
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 2
IP  - 1
DP  - 1991 Feb
TI  - Establishment of Schwann cell lines by oncogene transfer.
PG  - 6-12
AB  - Schwann cell cultures prepared from murine sciatic nerves (MuSN), rat sciatic 
      nerves (RSN) and murine dorsal root ganglia (MuDRG) were transformed by the 
      introduction of oncogenes (v-arc, c-Ha-ras, and v-mos). The transformation was 
      carried out by infection with helper-free retroviruses containing the oncogene 
      and the neomycin-resistant gene which were produced in psi-2 cells. After G418 
      selection and cloning of cells showing spindle shape morphology, six different 
      transformed cell clones, i.e., MuSN-arc, MuDRG-arc, MuDRG-ras, MuDRG-mos, 
      RSN-arc, and RSN-mos, were established. All of the clones were labeled with 
      antibodies to the S-100 protein and the laminin, which are specific markers for 
      Schwann cells. Fibronectin and vimentin were not detected in these cell clones, 
      in contrast to fibroblasts as 3T3 and Rat-2. These cell clones have been 
      maintained with characteristics of Schwann cells for over 18 months. When 
      RSN-mos, one of the Schwann cell clones, and rat retinas were cocultured direct 
      cellular interaction was demonstrated. Furthermore, by the addition of 
      conditioned medium of the RSN-mos, a prominent activity promoting neurite 
      outgrowth in PC12 pheochromocytoma cells was observed.
FAU - Amagai, T
AU  - Amagai T
AD  - Department of Immunology and Microbiology, Meiji College of Oriental Medicine, 
      Hiyoshi-cho, Kyoto, Japan 629-03.
FAU - Hitomi, S
AU  - Hitomi S
FAU - Sakai, Y
AU  - Sakai Y
FAU - Komatsubara-Takagi, S
AU  - Komatsubara-Takagi S
FAU - Stocking, C
AU  - Stocking C
FAU - Ostertag, W
AU  - Ostertag W
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
EDAT- 1991/02/01 00:00
MHDA- 1991/02/01 00:01
CRDT- 2009/11/17 06:00
PHST- 1990/11/29 00:00 [received]
PHST- 2009/11/17 06:00 [entrez]
PHST- 1991/02/01 00:00 [pubmed]
PHST- 1991/02/01 00:01 [medline]
AID - 1044-7431(91)90034-L [pii]
AID - 10.1016/1044-7431(91)90034-l [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 1991 Feb;2(1):6-12. doi: 10.1016/1044-7431(91)90034-l.