PMID- 19912880
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20191111
IS  - 1044-7431 (Print)
IS  - 1044-7431 (Linking)
VI  - 3
IP  - 4
DP  - 1992 Aug
TI  - Induction of FOS immunoreactivity in central accessory olfactory structures of 
      the female rat following exposure to conspecific males.
PG  - 360-9
AB  - Reproductive events in the female rat can be influenced by exposure to the odors 
      of conspecific males. Much evidence indicates that these pheromonal effects are 
      mediated by the accessory olfactory system (AOS); however, individual cells 
      within the AOS that are stimulated following exposure to male odors have not yet 
      been visualized. The present experiment was designed to determine the effect of 
      exposure to conspecific males and male odors on signal transduction in central 
      AOS neurons as measured by immunohistochemical detection of the induction of the 
      fos-like protein. AOS structures examined included the accessory olfactory bulb 
      (AOB), medial amygdala (mAMYG), and bed nucleus of the stria terminalis (BNST). 
      Due to its importance in the control of reproductive activities and its direct 
      link to the AOS, the ventromedial nucleus of the hypothalamus (VMH) was also 
      examined. Adult, ovariectomized rats were injected with estradiol benzoate (EB) 
      and 48 h later were placed in cages containing bedding material soiled by 
      conspecific males or placed in cages containing clean bedding material. After 
      exposure durations ranging from 10 to 180 min, the animals were sacrificed and 
      the brains were immunohistochemically processed for detection of fos-like 
      immunoreactivity. Another group of ovariectomized, EB-injected females was 
      repeatedly paired with conspeciflc males for 15 min followed by 15 min of rest. 
      Repeated matings were conducted over a 60-, 120-, or 180-min period while control 
      animals were repeatedly exposed to clean bedding material. Quantitative analysis 
      of the number of fos-immunopositive cells in the AOB revealed that continuous 
      exposure to male-soiled bedding or repeated mating resulted in significant 
      induction of foslike immunoreactivity compared to controls. Both treatments 
      produced similar numbers of fos-like immunoreactive cells in the mitral and 
      granule cell layers of the AOB. Fos induction was apparent after 60 min of 
      treatment but was more prominent at 120 and 180 min. In the mAMYG, BLAST, and 
      VMH, differences between the two treatments were noted. Exposure to male-soiled 
      bedding for 60 min produced scattered staining in the mAMYG, BLAST, and VMH, 
      whereas 60 min of repetitive mating resulted in a more dense distribution of 
      fos-like immunoreactive cells in these areas. Strikingly distinct patterns of 
      fos-like immunoreactive cells were observed in the mAMYG, BLAST, and VMH 
      following 120 or 180 min of repetitive mating. These patterns were not present in 
      animals exposed to male odors. The findings indicate that exposure of female rats 
      to reproductively relevant stimuli resulted in induction of fos-like 
      immunoreactivity within the AOS and that both olfactory and nonolfactory cues 
      probably contributed to this effect.
FAU - Dudley, C A
AU  - Dudley CA
AD  - University of Texas Southwestern Medical Center, Department of Physiology, 5323 
      Harry Hines Boulevard, Dallas, Texas 75235-9040, USA.
FAU - Rajendren, G
AU  - Rajendren G
FAU - Moss, R L
AU  - Moss RL
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Neurosci
JT  - Molecular and cellular neurosciences
JID - 9100095
EDAT- 1992/08/01 00:00
MHDA- 1992/08/01 00:01
CRDT- 2009/11/17 06:00
PHST- 1992/01/30 00:00 [received]
PHST- 2009/11/17 06:00 [entrez]
PHST- 1992/08/01 00:00 [pubmed]
PHST- 1992/08/01 00:01 [medline]
AID - 1044-7431(92)90034-Y [pii]
AID - 10.1016/1044-7431(92)90034-y [doi]
PST - ppublish
SO  - Mol Cell Neurosci. 1992 Aug;3(4):360-9. doi: 10.1016/1044-7431(92)90034-y.