PMID- 19913532 OWN - NLM STAT- MEDLINE DCOM- 20100225 LR - 20211028 IS - 1090-2422 (Electronic) IS - 0014-4827 (Print) IS - 0014-4827 (Linking) VI - 316 IP - 3 DP - 2010 Feb 1 TI - Amphiregulin mediates self-renewal in an immortal mammary epithelial cell line with stem cell characteristics. PG - 422-32 LID - 10.1016/j.yexcr.2009.11.006 [doi] AB - Amphiregulin (AREG), a ligand for epidermal growth factor receptor, is required for mammary gland ductal morphogenesis and mediates estrogen actions in vivo, emerging as an essential growth factor during mammary gland growth and differentiation. The COMMA-D beta-geo (CDbetageo) mouse mammary cell line displays characteristics of normal mammary progenitor cells including the ability to regenerate a mammary gland when transplanted into the cleared fat pad of a juvenile mouse, nuclear label retention, and the capacity to form anchorage-independent mammospheres. We demonstrate that AREG is essential for formation of floating mammospheres by CDbetageo cells and that the mitogen activated protein kinase signaling pathway is involved in AREG-mediated mammosphere formation. Addition of exogenous AREG promotes mammosphere formation in cells where AREG expression is knocked down by siRNA and mammosphere formation by AREG(-/-) mammary epithelial cells. AREG knockdown inhibits mammosphere formation by duct-limited mammary progenitor cells but not lobule-limited mammary progenitor cells. These data demonstrate AREG mediates the function of a subset of mammary progenitor cells in vitro. CI - Copyright 2009 Elsevier Inc. All rights reserved. FAU - Booth, Brian W AU - Booth BW AD - Mammary Biology and Tumorigenesis Laboratory, National Cancer Institute, National Institutes of Health, Bethesda, MD 20892, USA. brbooth@clemson.edu FAU - Boulanger, Corinne A AU - Boulanger CA FAU - Anderson, Lisa H AU - Anderson LH FAU - Jimenez-Rojo, Lucia AU - Jimenez-Rojo L FAU - Brisken, Cathrin AU - Brisken C FAU - Smith, Gilbert H AU - Smith GH LA - eng GR - Z99 CA999999/ImNIH/Intramural NIH HHS/United States PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20091112 PL - United States TA - Exp Cell Res JT - Experimental cell research JID - 0373226 RN - 0 (Amphiregulin) RN - 0 (Areg protein, mouse) RN - 0 (EGF Family of Proteins) RN - 0 (Glycoproteins) RN - 0 (Intercellular Signaling Peptides and Proteins) RN - 0 (RNA, Small Interfering) RN - EC 2.7.11.24 (Mitogen-Activated Protein Kinases) SB - IM MH - Amphiregulin MH - Animals MH - Cell Line, Transformed MH - Cell Proliferation MH - EGF Family of Proteins MH - Epithelial Cells/*cytology/enzymology/*metabolism MH - Female MH - Glycoproteins/antagonists & inhibitors/deficiency/*metabolism MH - Intercellular Signaling Peptides and Proteins/deficiency/*metabolism MH - Mammary Glands, Animal/*cytology MH - Mice MH - Mitogen-Activated Protein Kinases/metabolism MH - RNA, Small Interfering/metabolism MH - Stem Cells/*cytology/*metabolism MH - Transfection PMC - PMC2812656 MID - NIHMS163453 EDAT- 2009/11/17 06:00 MHDA- 2010/02/26 06:00 PMCR- 2011/02/01 CRDT- 2009/11/17 06:00 PHST- 2009/06/24 00:00 [received] PHST- 2009/11/04 00:00 [revised] PHST- 2009/11/09 00:00 [accepted] PHST- 2009/11/17 06:00 [entrez] PHST- 2009/11/17 06:00 [pubmed] PHST- 2010/02/26 06:00 [medline] PHST- 2011/02/01 00:00 [pmc-release] AID - S0014-4827(09)00503-5 [pii] AID - 10.1016/j.yexcr.2009.11.006 [doi] PST - ppublish SO - Exp Cell Res. 2010 Feb 1;316(3):422-32. doi: 10.1016/j.yexcr.2009.11.006. Epub 2009 Nov 12.