PMID- 19914247
OWN - NLM
STAT- MEDLINE
DCOM- 20100121
LR  - 20211203
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 584
IP  - 1
DP  - 2010 Jan 4
TI  - Opposing and uncoupling effects of mTOR and S6K1 in the regulation of endothelial 
      tissue factor expression.
PG  - 135-40
LID - 10.1016/j.febslet.2009.11.030 [doi]
AB  - Rapamycin has been reported to enhance tissue factor (TF) expression. The present 
      study investigated roles of mammalian target of rapamycin (mTOR) and its 
      downstream S6K1 in this process. We showed here that, consistent with rapamycin, 
      knocking-down mTOR enhanced thrombin-induced TF mRNA and protein levels, whereas 
      silencing S6K1 mitigated up-regulation of TF protein but not TF mRNA level. The 
      enhanced TF protein level upon mTOR-silencing was further augmented by 
      over-expression of a constitutively active S6K1 mutant and reduced by blocking 
      RhoA, p38(mapk) or NF-kappaB. The results reveal an opposing and uncoupling 
      effect of mTOR and S6K1 in regulating TF expression.
FAU - Ming, Xiu-Fen
AU  - Ming XF
AD  - Division of Physiology, Department of Medicine, University of Fribourg, Fribourg, 
      Switzerland. xiu-fen.ming@unifr.ch
FAU - Rajapakse, Angana Gupta
AU  - Rajapakse AG
FAU - Carvas, Joao Miguel
AU  - Carvas JM
FAU - Ruffieux, Jean
AU  - Ruffieux J
FAU - Yang, Zhihong
AU  - Yang Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (NF-kappa B)
RN  - 124671-05-2 (RHOA protein, human)
RN  - 9035-58-9 (Thromboplastin)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (RPS6KA1 protein, human)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 90-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.6.5.2 (rhoA GTP-Binding Protein)
SB  - IM
MH  - Adenoviridae
MH  - Cells, Cultured
MH  - Endothelium, Vascular/*metabolism
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - NF-kappa B/metabolism
MH  - Protein Kinases/genetics/*metabolism
MH  - RNA Interference
MH  - Ribosomal Protein S6 Kinases, 90-kDa/genetics/*metabolism
MH  - TOR Serine-Threonine Kinases
MH  - Thromboplastin/*biosynthesis/genetics
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
MH  - rhoA GTP-Binding Protein
EDAT- 2009/11/17 06:00
MHDA- 2010/01/22 06:00
CRDT- 2009/11/17 06:00
PHST- 2009/01/20 00:00 [received]
PHST- 2009/10/05 00:00 [revised]
PHST- 2009/11/09 00:00 [accepted]
PHST- 2009/11/17 06:00 [entrez]
PHST- 2009/11/17 06:00 [pubmed]
PHST- 2010/01/22 06:00 [medline]
AID - S0014-5793(09)00934-X [pii]
AID - 10.1016/j.febslet.2009.11.030 [doi]
PST - ppublish
SO  - FEBS Lett. 2010 Jan 4;584(1):135-40. doi: 10.1016/j.febslet.2009.11.030.