PMID- 19915049
OWN - NLM
STAT- MEDLINE
DCOM- 20091222
LR  - 20231006
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Linking)
VI  - 183
IP  - 11
DP  - 2009 Dec 1
TI  - Absence of the transcriptional repressor Blimp-1 in hematopoietic lineages 
      reveals its role in dendritic cell homeostatic development and function.
PG  - 7039-46
LID - 10.4049/jimmunol.0901543 [doi]
AB  - Dendritic cells (DCs) are important for the initiation and regulation of immune 
      responses. In this study, we demonstrate that DC homeostatic development in 
      peripheral lymphoid organs is negatively regulated by the transcriptional 
      repressor, Blimp-1, which is critical for regulation of plasma cell 
      differentiation and T cell homeostasis and function. Deletion of Prdm1, the gene 
      encoding Blimp-1, in mouse hematopoietic lineages resulted in an increase in the 
      steady-state number of conventional DCs (cDCs). Specifically, Prdm1 deletion 
      increased immediate CD8(-) cDC precursors in peripheral lymphoid organs, causing 
      selective expansion of the CD8(-) cDC population. Upon stimulus-induced 
      maturation, Blimp-1 was up-regulated in bone marrow-derived DCs via the p38 MAPK 
      and NF-kappaB pathways. Notably, Blimp-1-deficient DCs matured poorly upon 
      stimulation in vitro and in vivo. Blimp-1 binds to the proinflammatory 
      cytokine/chemokine genes, Il-6 and Ccl2, and negatively regulates their 
      expression. Collectively, our findings reveal two new roles for Blimp-1: negative 
      regulation of a select subset of cDCs during homeostatic development, and 
      enhancement of DC maturation.
FAU - Chan, Yueh-Hsuan
AU  - Chan YH
AD  - Graduate Institute of Life Sciences, National Defense Medical Center, Taipei, 
      Taiwan.
FAU - Chiang, Ming-Feng
AU  - Chiang MF
FAU - Tsai, Yueh-Chiao
AU  - Tsai YC
FAU - Su, Shin-Tang
AU  - Su ST
FAU - Chen, Ming-Hsu
AU  - Chen MH
FAU - Hou, Mau-Sheng
AU  - Hou MS
FAU - Lin, Kuo-I
AU  - Lin KI
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091113
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Prdm1 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - EC 2.1.1.- (Positive Regulatory Domain I-Binding Factor 1)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Cell Differentiation/*immunology
MH  - Cell Lineage/*immunology
MH  - Chromatin Immunoprecipitation
MH  - Dendritic Cells/*cytology/immunology/metabolism
MH  - Electrophoretic Mobility Shift Assay
MH  - Flow Cytometry
MH  - Fluorescent Antibody Technique
MH  - Homeostasis/*immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Positive Regulatory Domain I-Binding Factor 1
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Signal Transduction/genetics/immunology
MH  - Transcription Factors/genetics/*immunology/metabolism
EDAT- 2009/11/17 06:00
MHDA- 2009/12/23 06:00
CRDT- 2009/11/17 06:00
PHST- 2009/11/17 06:00 [entrez]
PHST- 2009/11/17 06:00 [pubmed]
PHST- 2009/12/23 06:00 [medline]
AID - jimmunol.0901543 [pii]
AID - 10.4049/jimmunol.0901543 [doi]
PST - ppublish
SO  - J Immunol. 2009 Dec 1;183(11):7039-46. doi: 10.4049/jimmunol.0901543. Epub 2009 
      Nov 13.