PMID- 19918608
OWN - NLM
STAT- MEDLINE
DCOM- 20100223
LR  - 20171116
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 134
IP  - 12
DP  - 2009 Dec
TI  - Electrochemical probing of HIV enzymes using ferrocene-conjugated peptides on 
      surfaces.
PG  - 2400-4
LID - 10.1039/b912083a [doi]
AB  - One of the current pathways to develop inhibitors that target different steps in 
      the life cycle of the human immunodeficiency virus (HIV) is blocking the function 
      of the HIV-related proteins such as HIV-1 integrase (HIV-1 IN), HIV-1 reverse 
      transcriptase (HIV-1 RT) and HIV-1 protease (HIV-1 PR), which are essential 
      proteins that control the ability of HIV to infect cells, produce new copies of 
      the virus, or cause disease. We have demonstrated for the first time the 
      detection of these enzymes at nanomolar levels using ferrocene (Fc)-conjugated 
      peptides on gold microelectrodes. The interaction between the Fc-conjugated 
      peptides and the enzymes was studied by cyclic voltammetry. As the protein 
      concentration increased, the electrochemical behaviour of the surface-bound Fc- 
      bioconjugate changed, indicating that HIV protein was binding to the peptide film 
      and encapsulating the Fc redox center on the surface. The electrochemical 
      responses shifted to higher potentials and decreased in the current intensity, as 
      the concentrations of the HIV-1 enzymes increased. The optimization studies were 
      performed by changing the pH and NaCl concentration. Control experiments involved 
      the exposure of the Fc-conjugated peptides with all the enzymes. This general 
      procedure can be readily applied in the future to the multiplexed detection of 
      several HIV-related proteins, as well as the high-throughput screening of 
      candidate inhibitors for AIDS therapy.
FAU - Kerman, Kagan
AU  - Kerman K
AD  - Department of Chemistry, The University of Western Ontario, 1265 Richmond Street, 
      London, ON, Canada N6A 5B7.
FAU - Kraatz, Heinz-Bernhard
AU  - Kraatz HB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091006
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Ferrous Compounds)
RN  - 0 (Human Immunodeficiency Virus Proteins)
RN  - 0 (Metallocenes)
RN  - 0 (Peptides)
RN  - U96PKG90JQ (ferrocene)
SB  - IM
MH  - Binding Sites
MH  - Electrochemical Techniques/*instrumentation/methods
MH  - Ferrous Compounds/*chemistry
MH  - HIV-1/*enzymology
MH  - Human Immunodeficiency Virus Proteins/*chemistry
MH  - Metallocenes
MH  - Nanotechnology
MH  - Peptides/*chemistry
MH  - Protein Binding
MH  - Surface Properties
EDAT- 2009/11/18 06:00
MHDA- 2010/02/24 06:00
CRDT- 2009/11/18 06:00
PHST- 2009/11/18 06:00 [entrez]
PHST- 2009/11/18 06:00 [pubmed]
PHST- 2010/02/24 06:00 [medline]
AID - 10.1039/b912083a [doi]
PST - ppublish
SO  - Analyst. 2009 Dec;134(12):2400-4. doi: 10.1039/b912083a. Epub 2009 Oct 6.