PMID- 19919178
OWN - NLM
STAT- MEDLINE
DCOM- 20100302
LR  - 20100112
IS  - 1437-4315 (Electronic)
IS  - 1431-6730 (Linking)
VI  - 391
IP  - 1
DP  - 2010 Jan
TI  - Potential role of multiple members of the kallikrein-related peptidase family of 
      serine proteases in activating latent TGF beta 1 in semen.
PG  - 85-95
LID - 10.1515/BC.2010.007 [doi]
AB  - Transforming growth factor beta1 (TGF beta 1) has been implicated as a key 
      contributor of immunosuppression in seminal plasma. The biochemical mechanisms 
      that lead to production of active seminal TGF beta 1 are not fully understood. It 
      is plausible that TGF beta 1 activation is partly induced simultaneously with the 
      release of motile spermatozoa following liquefaction of the semen coagulum. 
      Several members of the kallikrein-related peptidase (KLK) family are involved in 
      the regulation of semen liquefaction. This study examines the involvement of 
      these KLKs in TGF beta 1 activation in vitro and ex vivo, in seminal plasma. 
      Latent TGF beta 1 was rapidly activated by KLK14. The latency-associated 
      propeptide (LAP) was shown to be cleaved by KLK14 into small peptide fragments, 
      providing a possible mechanism for TGF beta 1 activation. KLK14 also cleaved the 
      latent TGFbeta binding protein 1 (LTBP1). KLK1, 2, and 5 might also contribute to 
      TGF beta 1 activation by nicking the LAP motif and inducing conformational 
      changes that aid in subsequent processing of LAP or through LTBP1 cleavage. Our 
      study provides strong evidence for the involvement of multiple members of the 
      seminal KLK cascade in activation of latent TGF beta 1 in seminal plasma. These 
      findings might have clinical implications in infertility treatment of cases with 
      concurrent delayed liquefaction and TGF beta 1-related semen antigenicity.
FAU - Emami, Nashmil
AU  - Emami N
AD  - Department of Laboratory Medicine and Pathobiology, University of Toronto, 
      Toronto M5G 1L5, Ontario, Canada.
FAU - Diamandis, Eleftherios P
AU  - Diamandis EP
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Biol Chem
JT  - Biological chemistry
JID - 9700112
RN  - 0 (LTBP1 protein, human)
RN  - 0 (Latent TGF-beta Binding Proteins)
RN  - 0 (Transforming Growth Factor beta1)
RN  - EC 3.4.21.- (KLK12 protein, human)
RN  - EC 3.4.21.- (KLK13 protein, human)
RN  - EC 3.4.21.- (KLK14 protein, human)
RN  - EC 3.4.21.- (Kallikreins)
SB  - IM
MH  - Humans
MH  - Kallikreins/metabolism/*physiology
MH  - Latent TGF-beta Binding Proteins/metabolism
MH  - Male
MH  - Semen/*metabolism
MH  - Transforming Growth Factor beta1/*metabolism
EDAT- 2009/11/19 06:00
MHDA- 2010/03/03 06:00
CRDT- 2009/11/19 06:00
PHST- 2009/11/19 06:00 [entrez]
PHST- 2009/11/19 06:00 [pubmed]
PHST- 2010/03/03 06:00 [medline]
AID - 10.1515/BC.2010.007 [doi]
PST - ppublish
SO  - Biol Chem. 2010 Jan;391(1):85-95. doi: 10.1515/BC.2010.007.