PMID- 19923073
OWN - NLM
STAT- MEDLINE
DCOM- 20110331
LR  - 20191210
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 29
IP  - 11
DP  - 2009 Nov
TI  - [Evaluation on parallel HER-2 testing using in situ hybridization and 
      immunohistochemistry in breast cancer tissues].
PG  - 2225-7
AB  - OBJECTIVE: To evaluate the application of the immunohistochemistry (IHC) and the 
      fluorescence in situ hybridization (FISH) in detecting the amplification and the 
      expression of HER-2 gene in the breast cancer patients. METHODS: Sixty-six cases 
      of paraffin-embeded breast cancer samples with overexpression, low or no 
      expression of HER-2 gene as detected by IHC were analyzed for HER-2 gene 
      amplification using FISH. RESULTS: Among the 42 samples with HER-2 gene 
      overexpression (3+/2+) detected by IHC, 31 showed positive HER-2 gene 
      amplification and 11 showed negative HER-2 gene amplification in FISH. In the 24 
      samples with low or no HER-2 gene expression (1+/-) detected by IHC, no HER-2 
      gene amplification was detected by FISH. The results of the two testing methods 
      showed a good consistency with the kappa coefficient of 0.672 (P<0.001). We also 
      found that the 17 chromosome polysomy in 42% of the samples and the incidence of 
      17 polysomy was significantly higher in the HER-2 gene overexpression (3+/2+) 
      group than in low or no HER-2 gene expression (1+/-) group (chi(2)=4.688, 
      P=0.03). CONCLUSION: IHC can be used as a screening method for detecting HER-2 
      gene amplification, and FISH should be performed in cases of HER-2 gene 
      overexpression (3+/2+) as detected by IHC.
FAU - Pan, Xiao-Ying
AU  - Pan XY
AD  - Center of Prenatal Diagnosis, Maternal and Children's Hospital of Guangdong 
      Province, Guangzhou 510010, China. panxiaoying5@yahoo.com.cn
FAU - Gao, Hong-Yi
AU  - Gao HY
FAU - Zhang, Jia-Li
AU  - Zhang JL
FAU - Fu, Wen-Ting
AU  - Fu WT
FAU - Liu, Shu
AU  - Liu S
LA  - chi
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - EC 2.7.10.1 (ERBB2 protein, human)
RN  - EC 2.7.10.1 (Receptor, ErbB-2)
SB  - IM
MH  - Breast Neoplasms/*genetics/metabolism
MH  - Carcinoma, Ductal, Breast/*genetics/metabolism
MH  - Female
MH  - Humans
MH  - *Immunohistochemistry
MH  - *In Situ Hybridization, Fluorescence
MH  - Receptor, ErbB-2/*analysis/genetics
EDAT- 2009/11/20 06:00
MHDA- 2011/04/01 06:00
CRDT- 2009/11/20 06:00
PHST- 2009/11/20 06:00 [entrez]
PHST- 2009/11/20 06:00 [pubmed]
PHST- 2011/04/01 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2009 Nov;29(11):2225-7.