PMID- 19923798
OWN - NLM
STAT- MEDLINE
DCOM- 20100607
LR  - 20161125
IS  - 1421-9670 (Electronic)
IS  - 0250-8095 (Linking)
VI  - 31
IP  - 2
DP  - 2010
TI  - JNK/ATF2 pathway is involved in iodinated contrast media-induced apoptosis.
PG  - 125-33
LID - 10.1159/000259899 [doi]
AB  - BACKGROUND/AIMS: Notably, activating transcriptional factor 2 (ATF2), a 
      histone-modification gene, is involved in oxidative stress-induced apoptosis. The 
      aim of this study was to clarify the role of ATF2 in contrast media-induced 
      nephropathy. METHODS: Human embryonic kidney 293T cells were treated with four 
      different contrast media:ionic high-osmolar diatrizoate; ionic low-osmolar 
      iothalamate; non-ionic low-osmolar iohexol, and non-ionic iso-osmolar iodixanol. 
      The mRNA expression of ATF2 was determined by real-time PCR. Short interfering 
      RNA was used to knock down ATF2 mRNA expression. Phosphorylation of ATF2 was 
      measured by Western blotting. Wistar rats were administered either diatrizoate or 
      a normal saline injection. Apoptosis in kidney tubular cells was determined by 
      the presence of positive TUNEL stain. RESULTS: Diatrizoate, iodixanol and 
      iothalamate, but not iohexol, induced the expression of ATF2 mRNA and 
      phosphorylation of ATF2 in 293T cells in a time-dependent manner. More apoptotic 
      cells were in diatrizoate-treated kidney cells than in the saline injection group 
      (p < 0.00001). Cell death was significantly increased by knockdown ATF2 
      expression in the presence of diatrizoate, indicating a protective role of ATF2 
      in contrast media-induced apoptosis. CONCLUSIONS: Differential activation of ATF2 
      by different contrast media provides a new insight into the mechanism and 
      prevention of contrast-induced nephropathy.
CI  - Copyright (c) 2009 S. Karger AG, Basel.
FAU - Lee, Hsiang-Chun
AU  - Lee HC
AD  - Division of Cardiology, Department of Internal Medicine, Kaohsiung Medical 
      University Hospital, No. 100 Tz-You 1st Road, Kaohsiung, Taiwan, ROC.
FAU - Sheu, Sheng-Hsiung
AU  - Sheu SH
FAU - Yen, Hsueh-Wei
AU  - Yen HW
FAU - Lai, Wen-Ter
AU  - Lai WT
FAU - Chang, Jan-Gowth
AU  - Chang JG
LA  - eng
PT  - Journal Article
DEP - 20091117
PL  - Switzerland
TA  - Am J Nephrol
JT  - American journal of nephrology
JID - 8109361
RN  - 0 (ATF2 protein, human)
RN  - 0 (Activating Transcription Factor 2)
RN  - 0 (Contrast Media)
RN  - 0 (Tetrazolium Salts)
RN  - 0 (Thiazoles)
RN  - 117-96-4 (Diatrizoate)
RN  - 9679TC07X4 (Iodine)
RN  - EC 2.7.12.2 (MAP Kinase Kinase 4)
RN  - EUY85H477I (thiazolyl blue)
SB  - IM
MH  - Activating Transcription Factor 2/*metabolism
MH  - Animals
MH  - *Apoptosis
MH  - Cell Survival
MH  - Contrast Media/*pharmacology
MH  - Diatrizoate/pharmacology
MH  - Humans
MH  - In Situ Nick-End Labeling
MH  - Iodine/*pharmacology
MH  - Kidney/metabolism/pathology
MH  - MAP Kinase Kinase 4/*metabolism
MH  - Models, Biological
MH  - Phosphorylation
MH  - Rats
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tetrazolium Salts/pharmacology
MH  - Thiazoles/pharmacology
EDAT- 2009/11/20 06:00
MHDA- 2010/06/09 06:00
CRDT- 2009/11/20 06:00
PHST- 2009/04/15 00:00 [received]
PHST- 2009/10/12 00:00 [accepted]
PHST- 2009/11/20 06:00 [entrez]
PHST- 2009/11/20 06:00 [pubmed]
PHST- 2010/06/09 06:00 [medline]
AID - 000259899 [pii]
AID - 10.1159/000259899 [doi]
PST - ppublish
SO  - Am J Nephrol. 2010;31(2):125-33. doi: 10.1159/000259899. Epub 2009 Nov 17.