PMID- 19924863
OWN - NLM
STAT- MEDLINE
DCOM- 20100224
LR  - 20091216
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 81
IP  - 24
DP  - 2009 Dec 15
TI  - Gas chromatography/atmospheric pressure chemical ionization-time of flight mass 
      spectrometry: analytical validation and applicability to metabolic profiling.
PG  - 10071-9
LID - 10.1021/ac9006073 [doi]
AB  - Gas Chromatography (GC)-Mass Spectrometry (MS) with Atmospheric Pressure (AP) 
      interface was introduced more than 30 years ago but never became a mainstream 
      technique, mainly because of technical difficulties and cost of instrumentation. 
      A recently introduced multipurpose AP source created the opportunity to 
      reconsider the importance of AP ionization for GC. Here, we present an analytical 
      evaluation of GC/APCI-MS showing the benefits of soft atmospheric pressure 
      chemical ionization for GC in combination with a Time of Flight (TOF) mass 
      analyzer. During this study, the complete analytical procedure was optimized and 
      evaluated with respect to characteristic analytical parameters, such as 
      repeatability, reproducibility, linearity, and detection limits. Limits of 
      detection (LOD) were found within the range from 11.8 to 72.5 nM depending on the 
      type of compound. The intraday and interday repeatability tests demonstrate 
      relative standard deviations (RSDs) of peak areas between 0.7%-2.1% and 3.8%-6.4% 
      correspondingly. Finally, we applied the developed method to the analysis of 
      human cerebrospinal fluid (CSF) samples to check the potential of this new 
      analytical combination for metabolic profiling.
FAU - Carrasco-Pancorbo, Alegria
AU  - Carrasco-Pancorbo A
AD  - Biomolecular Mass Spectrometry Unit, Department of Parasitology, LUMC, Leiden, 
      The Netherlands.
FAU - Nevedomskaya, Ekaterina
AU  - Nevedomskaya E
FAU - Arthen-Engeland, Thomas
AU  - Arthen-Engeland T
FAU - Zey, Thomas
AU  - Zey T
FAU - Zurek, Gabriela
AU  - Zurek G
FAU - Baessmann, Carsten
AU  - Baessmann C
FAU - Deelder, Andre M
AU  - Deelder AM
FAU - Mayboroda, Oleg A
AU  - Mayboroda OA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
SB  - IM
MH  - Atmospheric Pressure
MH  - Cerebrospinal Fluid/*chemistry/metabolism
MH  - Gas Chromatography-Mass Spectrometry
MH  - Humans
MH  - Reproducibility of Results
MH  - Time Factors
EDAT- 2009/11/21 06:00
MHDA- 2010/02/25 06:00
CRDT- 2009/11/21 06:00
PHST- 2009/11/21 06:00 [entrez]
PHST- 2009/11/21 06:00 [pubmed]
PHST- 2010/02/25 06:00 [medline]
AID - 10.1021/ac9006073 [doi]
PST - ppublish
SO  - Anal Chem. 2009 Dec 15;81(24):10071-9. doi: 10.1021/ac9006073.