PMID- 19925828
OWN - NLM
STAT- MEDLINE
DCOM- 20100611
LR  - 20220317
IS  - 1879-0542 (Electronic)
IS  - 0165-2478 (Linking)
VI  - 128
IP  - 1
DP  - 2010 Jan 18
TI  - Regionalization of pIgR expression in the mucosa of mouse small intestine.
PG  - 59-67
LID - 10.1016/j.imlet.2009.11.005 [doi]
AB  - Few reports exist on the differences in cell populations or immunological 
      functions between the proximal and distal segments of the small intestine (SI). 
      In the current contribution we analyzed the expression of the polymeric 
      immunoglobulin receptor (pIgR) and alpha chains as well as the density of 
      IgA-producing cells from the proximal and distal intestinal segments from Balb/c 
      mice. Furthermore, by using real-time RT-PCR we quantified the expression of 
      cytokines (TNF-alpha, IFN-gamma, IL-4 and TGF-beta), Toll-like receptor-4 
      (TLR-4), and the glucocorticoid receptor (GR) involved in pIgR expression in 
      intestinal epithelial cells (IEC). In this study, for the first time it has been 
      demonstrated that the expression of the pIgR as well as alpha chain was greater 
      in the proximal than the distal segment of the small intestine of normal mice. 
      Moreover, we found striking differences in the expression of cytokines at the 
      different intestinal compartments. Whereas the expression of TNF-alpha, IFN-gamma 
      and TGF-beta was higher in lamina propria lymphocytes (LPL) of the distal than 
      proximal segment, it was higher in IEC of the proximal than distal segment. In 
      contrast, the expression of the gene for IL-4 was higher in the LPL of the 
      proximal segment and the IEC of the distal segment. Although the overall 
      expression of TNF-alpha, IL-4, IFN-gamma and TGF-beta was higher in the whole 
      mucosa of the distal than proximal segment, we propose that cytokines produced by 
      epithelial cells (TNF-alpha, IFN-gamma and TGF-beta) autocrinally up-regulate the 
      expression of mRNA for the pIgR. Finally the expression of the GR was higher in 
      the proximal segment, while the expression of the gene for TLR-4 was 
      significantly higher in the IEC of the distal than proximal segment. The higher 
      expression of pIgR found in the proximal segment is probably related to the 
      effect on epithelial cells of the higher production of TNF-alpha, IFN-gamma and 
      TGF-beta, as well as the higher expression of the glucocorticoid receptors. The 
      increased expression of pIgR in the proximal segment appears primarily 
      responsible for the increased secretory IgA levels in the small intestine of 
      mice. These results confirm and extend previous findings supporting the 
      compartmentalization of the intestinal immune system.
FAU - Resendiz-Albor, Aldo A
AU  - Resendiz-Albor AA
AD  - Seccion de Estudios de Postgrado e Investigacion, Laboratorio de Inmunidad de 
      Mucosas, Escuela Superior de Medicina, Instituto Politecnico Nacional, Plan de 
      San Luis y Diaz Miron, Mexico, D.F., Mexico.
FAU - Reina-Garfias, Humberto
AU  - Reina-Garfias H
FAU - Rojas-Hernandez, Saul
AU  - Rojas-Hernandez S
FAU - Jarillo-Luna, Adriana
AU  - Jarillo-Luna A
FAU - Rivera-Aguilar, Victor
AU  - Rivera-Aguilar V
FAU - Miliar-Garcia, Angel
AU  - Miliar-Garcia A
FAU - Campos-Rodriguez, Rafael
AU  - Campos-Rodriguez R
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091117
PL  - Netherlands
TA  - Immunol Lett
JT  - Immunology letters
JID - 7910006
RN  - 0 (Antibodies)
RN  - 0 (Cytokines)
RN  - 0 (Immunoglobulin A)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 0 (Receptors, Polymeric Immunoglobulin)
SB  - IM
MH  - Animals
MH  - Antibodies
MH  - Apoptosis
MH  - B-Lymphocytes/immunology/metabolism/pathology
MH  - Cells, Cultured
MH  - Cytokines/metabolism
MH  - Immunization, Passive
MH  - Immunoglobulin A/immunology/*metabolism
MH  - Intestinal Mucosa/immunology/*metabolism/pathology
MH  - Intestine, Small/immunology/*metabolism
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Organ Specificity
MH  - Protein Structure, Tertiary/genetics
MH  - Rabbits
MH  - Receptors, Glucocorticoid/metabolism
MH  - Receptors, Polymeric Immunoglobulin/genetics/immunology/*metabolism
MH  - Signal Transduction
MH  - Spleen/immunology/metabolism/pathology
EDAT- 2009/11/21 06:00
MHDA- 2010/06/12 06:00
CRDT- 2009/11/21 06:00
PHST- 2009/03/31 00:00 [received]
PHST- 2009/10/20 00:00 [revised]
PHST- 2009/11/10 00:00 [accepted]
PHST- 2009/11/21 06:00 [entrez]
PHST- 2009/11/21 06:00 [pubmed]
PHST- 2010/06/12 06:00 [medline]
AID - S0165-2478(09)00274-0 [pii]
AID - 10.1016/j.imlet.2009.11.005 [doi]
PST - ppublish
SO  - Immunol Lett. 2010 Jan 18;128(1):59-67. doi: 10.1016/j.imlet.2009.11.005. Epub 
      2009 Nov 17.