PMID- 19928234
OWN - NLM
STAT- MEDLINE
DCOM- 20160423
LR  - 20190715
IS  - 1533-4880 (Print)
IS  - 1533-4880 (Linking)
VI  - 9
IP  - 9
DP  - 2009 Sep
TI  - Bienzyme based biosensing platform using functionalized carbon nanotubes.
PG  - 5411-6
AB  - This work presents a novel approach to design an amperometric transducer capable 
      of detecting glucose and hydrogen peroxide. The biosensor is based on a 
      bienzyme-channelling configuration, employing the enzymes glucose oxidase and 
      horseradish peroxidase, which were immobilized with Toluidine blue (TB) 
      functionalized multiwalled carbon nanotubes (MWNTs). TB was covalently 
      immobilized with carboxylic acid groups of the carbon nanotubes via carbodiimide 
      reaction. The MWNT/TB/GOD/HRP/Nf nanobiocomposite was prepared by mixing the GOx 
      and HRP solution with TB functionalized CNTs followed by mixing homogeneously 
      with Nafion. The MWNTiTB/GOD/HRP/Nf nanobiocomposite was characterized by 
      scanning electron microscopy. The performance of the MWNT/TB/GOD/HRP/Nf 
      nanobiocomposite modified electrode was examined by electrochemical impedance 
      spectroscopy and cyclic voltammetry. Linear calibrations were achieved upto 1.4 x 
      10(-7)-1.6 x 10(-3) M for glucose and 6.8 x 10(-8)-1.7 x 10(-3) M for H2O2. A 
      remarkable feature of this bienzyme electrode is the possibility to detect 
      glucose and H2O2 at very low applied potentials where the noise level and 
      interferences from other electro-active compounds are minimal.
FAU - Jeykumari, D R Shobha
AU  - Jeykumari DR
AD  - Department of Analytical Chemistry, School of Chemical Sciences, University of 
      Madras, Guindy Campus, Chennai 600025, India.
FAU - Narayanan, S Sriman
AU  - Narayanan SS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Nanosci Nanotechnol
JT  - Journal of nanoscience and nanotechnology
JID - 101088195
RN  - 0 (Nanotubes, Carbon)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.1.3.4 (Glucose Oxidase)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
SB  - IM
MH  - Biosensing Techniques
MH  - *Electrochemistry
MH  - Glucose Oxidase/chemistry
MH  - Horseradish Peroxidase/chemistry
MH  - Hydrogen Peroxide
MH  - *Nanotubes, Carbon/chemistry
EDAT- 2009/11/26 06:00
MHDA- 2016/04/24 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2016/04/24 06:00 [medline]
AID - 10.1166/jnn.2009.1169 [doi]
PST - ppublish
SO  - J Nanosci Nanotechnol. 2009 Sep;9(9):5411-6. doi: 10.1166/jnn.2009.1169.