PMID- 19928450
OWN - NLM
STAT- MEDLINE
DCOM- 20100323
LR  - 20191111
IS  - 0034-7744 (Print)
IS  - 0034-7744 (Linking)
VI  - 57
IP  - 3
DP  - 2009 Sep
TI  - Humoral response of paracoccidioidomycosis sera in hamsters with different 
      Venezuelan isolates.
PG  - 505-13
AB  - Humoral response of paracoccidioidomycosis sera in hamsters with different 
      Venezuelan isolates. Paracoccidioidomycosis (PCM) is a progressive systemic 
      mycosis caused by the fungus Paraccocidioides brasiliensis (Pb), endemic to 
      Venezuela and Latin America. In this study, eight different Venezuelan isolates 
      obtained from patients with PCM, were inoculated intraperitoneally in Syrian 
      hamsters (Cricetus auratus) and studied by immune-serum. Each strain was 
      collected by gently scraping the surface of the culture medium (Sabouraud 
      Dextrose Agar) and suspended in 3 ml of 0.15 M phosphate-buffered saline. The 
      antigen obtained was called Paraccocidioides brasiliensis Crude Antigen (CAP). 
      Immunoblotting results showed that the immune-sera from hamsters recognized at 
      least 3 bands: one over 200 kDa, and two of 80 and 15-20 kDa. This study suggests 
      that IgG anti-CAP can reveal a significant variability in the eight Venezuelan 
      isolates. Sera from 88 infected hamsters were evaluated by ELISA with eight 
      different CAPs and Western blot with CAP 37383. ELISA results showed that, the 
      antigen of the virulent isolate 37383 had the highest percentage (38%) of 
      positivity, while the nonvirulent isolate 1458 had the lowest one (13.6%). 
      Furthermore, scanning densitometry revealed that the isolate 37383 had less bands 
      than the non-virulent isolates. These results suggest that the ELISA test with 
      CAP 37383 can detect circulating antibodies, and that this virulent isolate may 
      be useful for the diagnosis of PCM, and to monitor disease responses to 
      treatments.
FAU - Spencer, Lilian M
AU  - Spencer LM
AD  - Departamento de Biologia Celular, Universidad Simon Bolivar, Caracas, Venezuela, 
      Apartado 89000, Caracas 1080, Venezuela. lspencer@usb.ve
FAU - Mateu, Guaniri
AU  - Mateu G
FAU - Magaldi, Sylvia
AU  - Magaldi S
FAU - Garcia, Fabiola
AU  - Garcia F
FAU - Mata-Essayag, Sofia
AU  - Mata-Essayag S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Costa Rica
TA  - Rev Biol Trop
JT  - Revista de biologia tropical
JID - 0404267
RN  - 0 (Antibodies, Fungal)
SB  - IM
MH  - Animals
MH  - Antibodies, Fungal/blood/*immunology
MH  - Cricetinae
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Immunity, Humoral/*immunology
MH  - Immunoblotting
MH  - Male
MH  - Mesocricetus
MH  - Paracoccidioides/*immunology/pathogenicity
MH  - Paracoccidioidomycosis/*immunology/microbiology
MH  - Venezuela
MH  - Virulence
EDAT- 2009/11/26 06:00
MHDA- 2010/03/24 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/03/24 06:00 [medline]
AID - 10.15517/rbt.v57i3.5471 [doi]
PST - ppublish
SO  - Rev Biol Trop. 2009 Sep;57(3):505-13. doi: 10.15517/rbt.v57i3.5471.