PMID- 19929553
OWN - NLM
STAT- MEDLINE
DCOM- 20100201
LR  - 20191210
IS  - 1437-4331 (Electronic)
IS  - 1434-6621 (Linking)
VI  - 47
IP  - 12
DP  - 2009
TI  - A new automated human leukocyte antigen genotyping strategy to identify DR-DQ 
      risk alleles for celiac disease and type 1 diabetes mellitus.
PG  - 1489-95
LID - 10.1515/CCLM.2009.346 [doi]
AB  - BACKGROUND: The risk for type 1 diabetes mellitus (T1DM) and celiac disease (CD) 
      is related to human leukocyte antigen (HLA) DQA1, DQB1 and DRB1 loci. 
      Unfortunately, HLA typing has been too difficult and costly for frequent use. 
      Automated genotyping focused on risk alleles could provide access to HLA typing 
      in diagnostic evaluations, epidemiological screening and contribute to preventive 
      strategies. METHODS: A sequence specific primer amplification method requiring a 
      total of four PCR reactions, one restriction enzyme digestion and a single 
      electrophoretic step provides low to medium resolution typing of DQA1, DQB1 and 
      DRB1 using Applied Biosystems 3730 DNA analyzer. The method was validated using 
      261 samples with genotypes determined using a reference method. RESULTS: Specific 
      fluorescent DQA1, DQB1 and DRB1 amplicons were of expected size. Concordance with 
      the reference method was 100% for DQA1 and DQB1 alleles and 99.8% for DRB1 
      alleles. CONCLUSIONS: We have developed a high throughput HLA typing method that 
      accurately distinguishes risk alleles for T1DM and CD. This method allows 
      screening of several thousand samples per week, consuming 32 ng of DNA template, 
      low reagent volumes and minimal time for data review.
FAU - Lavant, Ewa H
AU  - Lavant EH
AD  - Department of Biomedical Laboratory Science, Faculty of Health and Society, Malmo 
      University, Malmo, Sweden. Ewa.Lavant@skane.se
FAU - Carlson, Joyce A
AU  - Carlson JA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 0 (DNA Primers)
RN  - 0 (HLA-DQ Antigens)
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - *Alleles
MH  - *Automation
MH  - Base Sequence
MH  - Celiac Disease/*genetics
MH  - DNA Primers
MH  - Diabetes Mellitus, Type 1/*genetics
MH  - *Genetic Predisposition to Disease
MH  - Genotype
MH  - HLA-DQ Antigens/*genetics
MH  - HLA-DR Antigens/*genetics
MH  - Humans
MH  - Polymerase Chain Reaction
EDAT- 2009/11/26 06:00
MHDA- 2010/02/02 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/02/02 06:00 [medline]
AID - 10.1515/CCLM.2009.346 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2009;47(12):1489-95. doi: 10.1515/CCLM.2009.346.