PMID- 19930159
OWN - NLM
STAT- MEDLINE
DCOM- 20100316
LR  - 20151119
IS  - 1349-7006 (Electronic)
IS  - 1347-9032 (Linking)
VI  - 101
IP  - 2
DP  - 2010 Feb
TI  - Identification of transgelin-2 as a biomarker of colorectal cancer by laser 
      capture microdissection and quantitative proteome analysis.
PG  - 523-9
LID - 10.1111/j.1349-7006.2009.01424.x [doi]
AB  - To search for potential protein markers of colorectal cancer (CRC), the changes 
      in protein expression levels between microdissected tumor cells and normal mucosa 
      epithelia were analyzed by an acetylation stable isotopic labeling method coupled 
      with linear quadrupole ion trap fourier transform mass spectrometry (LTQ-FTMS). 
      In total, 137 proteins were up-regulated or down-regulated significantly in 
      cancer by at least two-fold. Based on gene ontology analysis, the largest part of 
      differential proteins were unknown for both subcellular localization and 
      biological process. In particular, the significant up-regulation of transgelin-2 
      (TAGLN2) in CRC was validated by Western blot analysis and further evaluated by 
      immunohistochemistry in paired tumor and normal mucosa samples from 120 
      consecutive CRC patients, 20 adenomas, and eight synchronous hepatic metastases 
      of CRC. TAGLN2 expression was frequently observed in cancer cells, precancerous 
      lesions, and hepatic metastases, whereas in normal epithelia expression was 
      rarely observed. The overexpression of TAGLN2 was associated with lymph node and 
      distant metastasis, advanced clinical stage (P < 0.001), and shorter overall 
      survival in CRCs. Cox regression analysis indicated that high tumor-TAGLN2 
      expression represents an independent prognostic factor. Consequently, 
      over-expression of TAGLN2 may serve as a new biomarker for predicting progression 
      and prognosis of CRC.
FAU - Zhang, Yanbin
AU  - Zhang Y
AD  - Department of Gastroenterological Surgery, Peking University People's Hospital, 
      Peking University, Beijing, China.
FAU - Ye, Yingjiang
AU  - Ye Y
FAU - Shen, Danhua
AU  - Shen D
FAU - Jiang, Kewei
AU  - Jiang K
FAU - Zhang, Hui
AU  - Zhang H
FAU - Sun, Wei
AU  - Sun W
FAU - Zhang, Jiyang
AU  - Zhang J
FAU - Xu, Feng
AU  - Xu F
FAU - Cui, Zhirong
AU  - Cui Z
FAU - Wang, Shan
AU  - Wang S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091103
PL  - England
TA  - Cancer Sci
JT  - Cancer science
JID - 101168776
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Microfilament Proteins)
RN  - 0 (Muscle Proteins)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Proteome)
RN  - 0 (transgelin)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Biomarkers, Tumor/*analysis
MH  - Colorectal Neoplasms/chemistry/*diagnosis
MH  - Female
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Microdissection
MH  - Microfilament Proteins/*analysis
MH  - Middle Aged
MH  - Muscle Proteins/*analysis
MH  - Neoplasm Proteins/*analysis
MH  - *Proteome
EDAT- 2009/11/26 06:00
MHDA- 2010/03/17 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/03/17 06:00 [medline]
AID - CAS1424 [pii]
AID - 10.1111/j.1349-7006.2009.01424.x [doi]
PST - ppublish
SO  - Cancer Sci. 2010 Feb;101(2):523-9. doi: 10.1111/j.1349-7006.2009.01424.x. Epub 
      2009 Nov 3.