PMID- 19931578
OWN - NLM
STAT- MEDLINE
DCOM- 20100628
LR  - 20181201
IS  - 1873-4863 (Electronic)
IS  - 0168-1656 (Linking)
VI  - 146
IP  - 4
DP  - 2010 Apr 15
TI  - Development and evaluation of rapid detection of classical swine fever virus by 
      reverse transcription loop-mediated isothermal amplification (RT-LAMP).
PG  - 147-50
LID - 10.1016/j.jbiotec.2009.11.006 [doi]
AB  - A one-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) 
      was developed for the detection of classical swine fever virus (CSFV) and a set 
      of primers designed based on the E2 gene reference sequences of the CSFV. The 
      assay was optimized to amplify CSFV RNA by incubation at 63 degrees C for 50 min. 
      The RT-LAMP amplification products had a ladder-like appearance when 
      electrophoresed on an agarose gel. The RT-LAMP assay showed higher sensitivity 
      than the conventional RT-PCR, and no cross-reactivity appeared with other related 
      porcine viruses including porcine circovirus type 2 (PCV2), porcine parvovirus 
      (PPV), porcine pseudorabies virus (PRV), and porcine reproductive and respiratory 
      syndrome virus (PRRSV). The positive predictive value (PPV) of CSFV RT-LAMP for 
      227 field tissue samples was 94.7%, the negative predictive value (NPV) was 30.8% 
      and showed better for the conventional RT-PCR method. Thus, the RT-LAMP assay is 
      extremely rapid, sensitive, and specific and has potential usefulness for rapid 
      laboratory diagnosis and pen-side detection for CSFV detection in pigs.
CI  - (c) 2009. Published by Elsevier B.V. All rights reserved.
FAU - Yin, Shuanghui
AU  - Yin S
AD  - Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      State Key Laboratory of Veterinary Etiological Biology, Key Laboratory of Animal 
      Virology of Ministry of Agriculture, Xujiaping No. 1, Lanzhou 730046, Gansu, 
      China.
FAU - Shang, Youjun
AU  - Shang Y
FAU - Zhou, Guangqing
AU  - Zhou G
FAU - Tian, Hong
AU  - Tian H
FAU - Liu, Yanhong
AU  - Liu Y
FAU - Cai, Xuepeng
AU  - Cai X
FAU - Liu, Xiangtao
AU  - Liu X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091201
PL  - Netherlands
TA  - J Biotechnol
JT  - Journal of biotechnology
JID - 8411927
RN  - 0 (RNA, Viral)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (glycoprotein E2, classical swine fever virus)
SB  - IM
MH  - Animals
MH  - Classical Swine Fever Virus/genetics/*isolation & purification
MH  - Nucleic Acid Amplification Techniques/*methods
MH  - Organ Specificity
MH  - RNA, Viral/genetics/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Viral Envelope Proteins/genetics
EDAT- 2009/11/26 06:00
MHDA- 2010/06/29 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/02/27 00:00 [received]
PHST- 2009/09/30 00:00 [revised]
PHST- 2009/11/09 00:00 [accepted]
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/06/29 06:00 [medline]
AID - S0168-1656(09)00527-6 [pii]
AID - 10.1016/j.jbiotec.2009.11.006 [doi]
PST - ppublish
SO  - J Biotechnol. 2010 Apr 15;146(4):147-50. doi: 10.1016/j.jbiotec.2009.11.006. Epub 
      2009 Dec 1.