PMID- 19931636
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20191210
IS  - 1531-4332 (Electronic)
IS  - 1095-6433 (Linking)
VI  - 155
IP  - 3
DP  - 2010 Mar
TI  - Na+/K+-ATPase and vacuolar-type H+-ATPase in the gills of the aquatic 
      air-breathing fish Trichogaster microlepis in response to salinity variation.
PG  - 309-18
LID - 10.1016/j.cbpa.2009.11.010 [doi]
AB  - The aquatic air-breathing fish, Trichogaster microlepis, can be found in fresh 
      water and estuaries. We further evaluated the changes in two important 
      osmoregulatory enzymes, Na(+)/K(+)-ATPase (NKA) and vacuolar-type H(+)-ATPase 
      (VHA), in the gills when fish were subjected to deionized water (DW), fresh water 
      (FW), and salinated brackish water (salinity of 10 g/L). Fish were sampled only 4 
      days after experimental transfer. The mortality, plasma osmolality, and Na(+) 
      concentration were higher in 10 g/L acclimated fish, while their muscle water 
      content decreased with elevated external salinity. The highest NKA protein 
      abundance was found in the fish gills in 10 g/L, and NKA activity was highest in 
      the DW and 10 g/L acclimated fish. The VHA protein levels were highest in 10 g/L, 
      and VHA activity was highest in the DW treatment. From immunohistochemical 
      results, we found three different cell populations: (1) NKA-immunoreactive 
      (NKA-IR) cells, (2) both NKA-IR and HA-IR cells, and (3) HA-IR cells. NKA-IR 
      cells in the lamellar and interlamellar regions significantly increased in DW and 
      10 g/L treatments. Only HA-IR cells in the lamellar region were significantly 
      increased in DW. In the interlamellar region, there was no difference in the 
      number of HA-IR cells among the three treated. From these results, T. microlepis 
      exhibited osmoregulatory ability in DW and 10 g/L treatments. The cell types 
      involved in ionic regulation were also examined with immunofluorescence staining; 
      three ionocyte types were found which were similar to the zebrafish model.
CI  - Copyright 2009 Elsevier B.V. All rights reserved.
FAU - Huang, Chun-Yen
AU  - Huang CY
AD  - Department of Life Science, Tunghai University, Taichung 40704, Taiwan.
FAU - Chao, Pei-Lin
AU  - Chao PL
FAU - Lin, Hui-Chen
AU  - Lin HC
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091118
PL  - United States
TA  - Comp Biochem Physiol A Mol Integr Physiol
JT  - Comparative biochemistry and physiology. Part A, Molecular & integrative 
      physiology
JID - 9806096
RN  - 0 (Fish Proteins)
RN  - 0 (Sodium-Potassium-Chloride Symporters)
RN  - 059QF0KO0R (Water)
RN  - EC 3.6.1.- (Vacuolar Proton-Translocating ATPases)
RN  - EC 7.2.2.13 (Sodium-Potassium-Exchanging ATPase)
SB  - IM
MH  - *Air
MH  - Animals
MH  - Fish Proteins/metabolism
MH  - Fishes/blood/*physiology
MH  - Fluorescent Antibody Technique
MH  - Gills/cytology/*enzymology
MH  - Immunoblotting
MH  - Microscopy, Confocal
MH  - Muscles/metabolism
MH  - Osmolar Concentration
MH  - Oxygen Consumption/*physiology
MH  - *Salinity
MH  - Sodium-Potassium-Chloride Symporters/metabolism
MH  - Sodium-Potassium-Exchanging ATPase/*metabolism
MH  - Survival Analysis
MH  - Vacuolar Proton-Translocating ATPases/*metabolism
MH  - Water/metabolism
EDAT- 2009/11/26 06:00
MHDA- 2010/04/14 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/09/20 00:00 [received]
PHST- 2009/11/10 00:00 [revised]
PHST- 2009/11/10 00:00 [accepted]
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - S1095-6433(09)01174-X [pii]
AID - 10.1016/j.cbpa.2009.11.010 [doi]
PST - ppublish
SO  - Comp Biochem Physiol A Mol Integr Physiol. 2010 Mar;155(3):309-18. doi: 
      10.1016/j.cbpa.2009.11.010. Epub 2009 Nov 18.