PMID- 19932849
OWN - NLM
STAT- MEDLINE
DCOM- 20100107
LR  - 20141120
IS  - 1097-6817 (Electronic)
IS  - 0194-5998 (Linking)
VI  - 141
IP  - 6
DP  - 2009 Dec
TI  - Immunologic response to fungus is not universally associated with rhinosinusitis.
PG  - 750-6.e1-2
LID - 10.1016/j.otohns.2009.09.016 [doi]
AB  - OBJECTIVE: Immunologic response to fungal antigens has been cited as an etiologic 
      factor in chronic rhinosinusitis (CRS). Previous work demonstrated a significant 
      cytokine response in CRS patients that did not correlate with an immunoglobulin E 
      (IgE) response. This study was performed in an effort to replicate these findings 
      in a more geographically diverse population. DESIGN: Prospective in vitro study. 
      SETTING: Two academic tertiary rhinologic practices in Texas and Utah. METHODS: 
      Serum and peripheral blood monocytes (PBMC) were obtained from 10 CRS patients 
      and seven controls. Total IgE and fungal-specific IgE levels were determined. 
      Cytokine levels were measured after PBMC exposure to Alternaria, Aspergillus, 
      Cladosporium, and Penicillium extracts. Correlations between cytokine responses 
      and presence of CRS as well as IgE and IgG were determined. RESULTS: 
      Interleukin-5 (IL-5) was produced after Alternaria extract exposure in both CRS 
      patients and controls, but the production was heterogenous and did not correlate 
      with the presence of CRS. IL-5 levels after Alternaria extract exposure 
      correlated strongly with levels of Alternaria-specific IgE in both CRS patients 
      and controls. IL-5 production did not correlate with IgG levels. IL-4, IL-13, and 
      interferon-gamma production did not differ between CRS patients and controls. 
      CONCLUSIONS: In contrast to previously reported data, IL-5 responses to 
      Alternaria extract were not predictive of CRS presence. Our results in patients 
      from Utah and Texas significantly differ from previously published findings in 
      predominantly Midwestern patients. The immunologic response to fungal extracts 
      appears to be heterogenous and may differ based on geography, allergy status, 
      and/or other as-yet unknown factors.
FAU - Orlandi, Richard R
AU  - Orlandi RR
AD  - Division of Otolaryngology-Head and Neck Surgery, University of Utah, Salt Lake 
      City, Utah, USA. richard.orlandi@hsc.utah.edu
FAU - Marple, Bradley F
AU  - Marple BF
FAU - Georgelas, Ann
AU  - Georgelas A
FAU - Durtschi, Drew
AU  - Durtschi D
FAU - Barr, Lucy
AU  - Barr L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Otolaryngol Head Neck Surg
JT  - Otolaryngology--head and neck surgery : official journal of American Academy of 
      Otolaryngology-Head and Neck Surgery
JID - 8508176
RN  - 0 (Antigens, Fungal)
RN  - 0 (Immunoglobulin G)
RN  - 0 (Interleukin-5)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
CIN - Otolaryngol Head Neck Surg. 2010 Nov;143(5):607-10. PMID: 20974326
MH  - Adult
MH  - Aged
MH  - Antigens, Fungal/*immunology
MH  - Chronic Disease
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Immunoglobulin G/blood
MH  - In Vitro Techniques
MH  - Interleukin-5/immunology
MH  - Male
MH  - Middle Aged
MH  - Prospective Studies
MH  - Rhinitis/*immunology
MH  - Sinusitis/*immunology
MH  - Texas
MH  - Utah
EDAT- 2009/11/26 06:00
MHDA- 2010/01/08 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/08/31 00:00 [received]
PHST- 2009/09/21 00:00 [accepted]
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/01/08 06:00 [medline]
AID - S0194-5998(09)01511-3 [pii]
AID - 10.1016/j.otohns.2009.09.016 [doi]
PST - ppublish
SO  - Otolaryngol Head Neck Surg. 2009 Dec;141(6):750-6.e1-2. doi: 
      10.1016/j.otohns.2009.09.016.