PMID- 19933266
OWN - NLM
STAT- MEDLINE
DCOM- 20100416
LR  - 20220331
IS  - 1471-9053 (Electronic)
IS  - 0032-0781 (Linking)
VI  - 51
IP  - 1
DP  - 2010 Jan
TI  - Knock-down of OsDCL2 in rice negatively affects maintenance of the endogenous 
      dsRNA virus, Oryza sativa endornavirus.
PG  - 58-67
LID - 10.1093/pcp/pcp167 [doi]
AB  - An endogenous double-stranded RNA (dsRNA), which has recently been recognized as 
      the dsRNA virus Oryza sativa endornavirus (OsEV), is found in many strains of 
      cultivated rice (Oryza sativa). Small RNAs derived from OsEV dsRNA were detected, 
      indicating that the RNA silencing machinery recognizes OsEV dsRNA. The existence 
      of OsEV in knock-down (KD) lines of five genes of RNA-dependent RNA polymerase 
      (OsRDR1-OsRDR5) or two genes of Dicer-like protein (OsDCL2 or OsDCL3a) was 
      examined to characterize the relationship between the host RNA silencing system 
      and the propagation of this dsRNA virus. OsEV was not detected in OsRDR4-KD or 
      OsDCL2-KD T(1) lines. We attempted to introduce OsEV into these KD lines by 
      crossing them with OsEV-carrying plants because of the efficient transmission of 
      OsEV to F(1) plants via pollen or ova. All OsRDR4-KD but only some OsDCL2-KD F(1) 
      plants contained OsEV. Some OsDCL2-KD F(1) plants consisted of OsEV-carrying and 
      OsEV-free cells. These results suggest that the maintenance of OsEV is unstable 
      in OsDCL2-KD plants. Furthermore, the amount of OsEV-derived small interfering 
      RNA (vsiRNA) in the OsDCL2-KD plants increased relative to the wild type. This 
      increased level of vsiRNA may cause OsEV instability during cell division.
FAU - Urayama, Syunichi
AU  - Urayama S
AD  - Department of Applied Biological Sciences, Tokyo University of Agriculture and 
      Technology, Tokyo, Japan.
FAU - Moriyama, Hiromitsu
AU  - Moriyama H
FAU - Aoki, Nanako
AU  - Aoki N
FAU - Nakazawa, Yukihiro
AU  - Nakazawa Y
FAU - Okada, Ryo
AU  - Okada R
FAU - Kiyota, Eri
AU  - Kiyota E
FAU - Miki, Daisuke
AU  - Miki D
FAU - Shimamoto, Ko
AU  - Shimamoto K
FAU - Fukuhara, Toshiyuki
AU  - Fukuhara T
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091119
PL  - Japan
TA  - Plant Cell Physiol
JT  - Plant & cell physiology
JID - 9430925
RN  - 0 (Arabidopsis Proteins)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (RNA, Double-Stranded)
RN  - 0 (RNA, Small Interfering)
RN  - EC 3.1.26.3 (DCL2 protein, Arabidopsis)
RN  - EC 3.1.26.3 (Ribonuclease III)
SB  - IM
MH  - Arabidopsis Proteins/genetics/metabolism
MH  - Cell Cycle Proteins/genetics/metabolism
MH  - Disease Transmission, Infectious
MH  - Down-Regulation/genetics
MH  - Gene Expression Regulation, Plant/genetics
MH  - Oryza/*genetics/metabolism/*virology
MH  - Plant Viruses/*genetics/metabolism
MH  - RNA Interference/*physiology
MH  - RNA Stability/genetics
MH  - RNA, Double-Stranded/*genetics
MH  - RNA, Small Interfering/genetics
MH  - Ribonuclease III/*genetics/metabolism
MH  - Virus Replication/genetics
EDAT- 2009/11/26 06:00
MHDA- 2010/04/17 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/04/17 06:00 [medline]
AID - pcp167 [pii]
AID - 10.1093/pcp/pcp167 [doi]
PST - ppublish
SO  - Plant Cell Physiol. 2010 Jan;51(1):58-67. doi: 10.1093/pcp/pcp167. Epub 2009 Nov 
      19.