PMID- 19933497
OWN - NLM
STAT- MEDLINE
DCOM- 20100422
LR  - 20131121
IS  - 1933-7205 (Electronic)
IS  - 1933-7191 (Linking)
VI  - 17
IP  - 3
DP  - 2010 Mar
TI  - Regulation of monocyte chemotactic protein-1 expression in human endometrial 
      endothelial cells by sex steroids: a potential mechanism for leukocyte 
      recruitment in endometriosis.
PG  - 278-87
LID - 10.1177/1933719109352380 [doi]
AB  - The main aim of this study is to describe the in vivo temporal and spatial 
      expression of monocyte chemotactic protein 1 (MCP-1) in human endometrial 
      endothelial cells (HEECs) and to compare the in vitro regulation of MCP-1 
      expression by sex steroids in HEECs from women with or without endometriosis. 
      Eutopic endometrial tissues and endometriosis implants were grouped according to 
      the menstrual cycle phase and were examined by immunohistochemistry for MCP-1 
      expression. No significant difference was observed for MCP-1 immunoreactivity in 
      the endothelial cells of eutopic endometrium of women with endometriosis when 
      compared to endometrium of women without endometriosis and to endometriosis 
      implants. For in vitro studies, the purity of cultured HEECs (90%-95%) was 
      confirmed by immunocytochemistry using endothelium-specific markers CD31 and 
      CD146. The effects of estradiol (5 x 10(- 8) mol/L), progesterone (10(-7) mol/L), 
      or both on MCP-1 messenger RNA (mRNA) and protein levels were analyzed by reverse 
      transcriptase-polymerase chain reaction (RT-PCR) analysis and enzyme-linked 
      immunosorbent serologic assay (ELISA), respectively. Sex steroids did not have 
      significant effect on MCP-1 mRNA and protein expression in HEECs from women 
      without endometriosis. However, we observed that the sex steroid treatment 
      stimulated MCP-1 mRNA and protein expression in HEECs from women with 
      endometriosis (P < .05). We postulate that the stimulation of chemokine 
      expression by sex steroids in the endometrial endothelial cells in women with 
      endometriosis may play a central role in recruiting mononuclear cells, therefore 
      contributing to the inflammatory aspect of endometriosis.
FAU - Luk, Janelle
AU  - Luk J
AD  - Section of Reproductive Endocrinology and Infertility, Department of Obstetrics, 
      Gynecology and Reproductive Sciences, Yale University School of Medicine, New 
      Haven, CT 06520, USA.
FAU - Seval, Yasemin
AU  - Seval Y
FAU - Ulukus, Murat
AU  - Ulukus M
FAU - Ulukus, Emine Cagnur
AU  - Ulukus EC
FAU - Arici, Aydin
AU  - Arici A
FAU - Kayisli, Umit A
AU  - Kayisli UA
LA  - eng
PT  - Journal Article
DEP - 20091120
PL  - United States
TA  - Reprod Sci
JT  - Reproductive sciences (Thousand Oaks, Calif.)
JID - 101291249
RN  - 0 (Chemokine CCL2)
RN  - 0 (Gonadal Steroid Hormones)
RN  - 0 (RNA, Messenger)
RN  - 4G7DS2Q64Y (Progesterone)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Adult
MH  - Cells, Cultured
MH  - Chemokine CCL2/analysis/*genetics
MH  - Endometriosis/immunology/*metabolism
MH  - Endometrium/*chemistry
MH  - Endothelial Cells/chemistry
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Estradiol/pharmacology
MH  - Female
MH  - Gene Expression Regulation/*drug effects
MH  - Gonadal Steroid Hormones/*pharmacology
MH  - Humans
MH  - Immunohistochemistry
MH  - Leukocytes/*physiology
MH  - Middle Aged
MH  - Progesterone/pharmacology
MH  - RNA, Messenger/analysis
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2009/11/26 06:00
MHDA- 2010/04/23 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/04/23 06:00 [medline]
AID - 1933719109352380 [pii]
AID - 10.1177/1933719109352380 [doi]
PST - ppublish
SO  - Reprod Sci. 2010 Mar;17(3):278-87. doi: 10.1177/1933719109352380. Epub 2009 Nov 
      20.