PMID- 19934596 OWN - NLM STAT- MEDLINE DCOM- 20100224 LR - 20211020 IS - 1976-555X (Electronic) IS - 1229-845X (Print) IS - 1229-845X (Linking) VI - 10 IP - 4 DP - 2009 Dec TI - Phylogenetic characterization of genes encoding for glycoprotein 5 and membrane protein of PRRSV isolate HH08. PG - 309-15 AB - A porcine reproductive and respiratory syndrome virus (PRRSV) was obtained from clinic samples. Genes 5 and 6 encoding for the viral glycoprotein 5 and a membrane protein of the PRRSV designated as HH08 were amplified by reverse transcription-PCR. These sequences were compared with reference sequences derived from different geographical locations. The results indicated that the virus belongs to the North American type rather than European. Comparative analyses of the genetic diversity between the PRRSV isolate HH08 and other Chinese as well as foreign reference strains of PRRSV were discussed based on the sequence comparison and the topology of phylogenetic trees constructed in this study. FAU - Wang, Mingcui AU - Wang M AD - College of Veterinary Medicine, Northeast Agricultural University, 59 Mucai Street, Xiangfang District, 150030 Harbin, China. FAU - Li, Guangxing AU - Li G FAU - Yin, Jiechao AU - Yin J FAU - Ren, Xiaofeng AU - Ren X LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Korea (South) TA - J Vet Sci JT - Journal of veterinary science JID - 100964185 RN - 0 (Viral Envelope Proteins) RN - 0 (Viral Matrix Proteins) RN - 0 (glycoprotein 5, PRRSV) SB - IM MH - Animals MH - Base Sequence MH - China/epidemiology MH - Gene Expression Regulation, Viral/physiology MH - Genetic Variation MH - Molecular Sequence Data MH - *Phylogeny MH - Porcine Reproductive and Respiratory Syndrome/epidemiology/virology MH - Porcine respiratory and reproductive syndrome virus/*genetics/*metabolism MH - Sequence Alignment MH - Swine MH - Viral Envelope Proteins/genetics/*metabolism MH - Viral Matrix Proteins/genetics/*metabolism PMC - PMC2807267 EDAT- 2009/11/26 06:00 MHDA- 2010/02/25 06:00 PMCR- 2009/12/01 CRDT- 2009/11/26 06:00 PHST- 2009/11/26 06:00 [entrez] PHST- 2009/11/26 06:00 [pubmed] PHST- 2010/02/25 06:00 [medline] PHST- 2009/12/01 00:00 [pmc-release] AID - 200912309 [pii] AID - 10.4142/jvs.2009.10.4.309 [doi] PST - ppublish SO - J Vet Sci. 2009 Dec;10(4):309-15. doi: 10.4142/jvs.2009.10.4.309.