PMID- 19935913
OWN - NLM
STAT- MEDLINE
DCOM- 20100308
LR  - 20161020
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 52
IP  - 11
DP  - 2009 Nov
TI  - Telomere analysis of platyhelminths and acanthocephalans by FISH and Southern 
      hybridization.
PG  - 897-903
LID - 10.1139/g09-063 [doi]
AB  - We examined the composition of telomeres in chromosomes of parasitic worms, 
      representatives of the flatworm groups Monogenea and Cestoda (Platyhelminthes), 
      and thorny-headed worms (Syndermata: Acanthocephala) by fluorescence in situ 
      hybridization (FISH) with different telomeric repeat probes. Our results show 
      that the (TTAGGG)n sequence, supposed to be the ancestral telomeric repeat motif 
      of Metazoa, is conserved in Monogenea (Paradiplozoon homoion) and Cestoda 
      (Caryophyllaeus laticeps, Caryophyllaeides fennica, and Nippotaenia mogurndae) 
      but not in Acanthocephala (Pomphorhynchus laevis and Pomphorhynchus 
      tereticollis). In the Pomphorhynchus species, no hybridization signals were 
      obtained with the "nematode" (TTAGGC)n, "arthropod" (TTAGG)n, and bdelloid 
      (TGTGGG)n telomeric probes using FISH with their chromosomes and Southern 
      hybridization with P. laevis DNA. Therefore, we suggest that parasitic 
      Acanthocephala have evolved yet unknown telomeric repeat motifs or different 
      mechanisms of telomere maintenance.
FAU - Bombarova, Marta
AU  - Bombarova M
AD  - Parasitological Institute, Slovak Academy of Sciences, Hlinkova 3, 04001 Kosice, 
      Slovakia.
FAU - Vitkova, Magda
AU  - Vitkova M
FAU - Spakulova, Marta
AU  - Spakulova M
FAU - Koubkova, Bozena
AU  - Koubkova B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - Acanthocephala/*genetics
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Southern
MH  - In Situ Hybridization, Fluorescence
MH  - Platyhelminths/*genetics
MH  - Telomere/*genetics
EDAT- 2009/11/26 06:00
MHDA- 2010/03/10 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/03/10 06:00 [medline]
AID - g09-063 [pii]
AID - 10.1139/g09-063 [doi]
PST - ppublish
SO  - Genome. 2009 Nov;52(11):897-903. doi: 10.1139/g09-063.