PMID- 19935922 OWN - NLM STAT- MEDLINE DCOM- 20100305 LR - 20220310 IS - 1480-3321 (Electronic) IS - 0831-2796 (Linking) VI - 52 IP - 9 DP - 2009 Sep TI - Physical mapping of a 7A.7D translocation in the wheat-Thinopyrum ponticum partial amphiploid BE-1 using multicolour genomic in situ hybridization and microsatellite marker analysis. PG - 748-54 LID - 10.1139/g09-047 [doi] AB - The absence of chromosome 7D in the wheat-Thinopyrum ponticum partial amphiploid BE-1 was detected previously by multicolour genomic in situ hybridization, sequential FISH (fluorescence in situ hybridization) using repetitive DNA probes, and SSR marker analysis. In the present study the previous cytogenetic and SSR marker analyses were expanded to include 25 other SSR markers assigned to wheat chromosomes 7A and 7D to confirm the presence of a 7A.7D translocation and to specify its composition. An almost complete chromosome 7A and a short chromosome segment derived from the terminal region of 7DL were detected, confirming the presence of a terminal translocation involving the distal regions of 7AL and 7DL. In both cases the position of the translocation breakpoint was different from that of known deletion lines. The identification of the 7AL.7DL translocation and its breakpoint position provides a new physical landmark for future physical mapping studies, opening up the possibility of more precise localization of genes or molecular markers within the terminal regions of 7DL and 7AL. FAU - Sepsi, A AU - Sepsi A AD - Agricultural Research Institute of the Hungarian Academy of Sciences, H-2462, Martonvasar, P.O. Box 19, Hungary. FAU - Molnar, I AU - Molnar I FAU - Molnar-Lang, M AU - Molnar-Lang M LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - Canada TA - Genome JT - Genome JID - 8704544 RN - 0 (DNA Probes) RN - 0 (DNA, Plant) SB - IM MH - Chromosome Painting/*methods MH - Chromosomes, Plant/*genetics MH - DNA Probes/genetics MH - DNA, Plant/genetics MH - Diploidy MH - Genome, Plant MH - Hybridization, Genetic MH - Microsatellite Repeats/*genetics MH - Physical Chromosome Mapping/*methods MH - Polymerase Chain Reaction MH - Translocation, Genetic EDAT- 2009/11/26 06:00 MHDA- 2010/03/06 06:00 CRDT- 2009/11/26 06:00 PHST- 2009/11/26 06:00 [entrez] PHST- 2009/11/26 06:00 [pubmed] PHST- 2010/03/06 06:00 [medline] AID - g09-047 [pii] AID - 10.1139/g09-047 [doi] PST - ppublish SO - Genome. 2009 Sep;52(9):748-54. doi: 10.1139/g09-047.