PMID- 19936900
OWN - NLM
STAT- MEDLINE
DCOM- 20100305
LR  - 20211020
IS  - 1875-8355 (Electronic)
IS  - 1572-3887 (Linking)
VI  - 29
IP  - 1
DP  - 2010 Jan
TI  - Comparative analysis of the effects of alpha-crystallin and GroEL on the kinetics 
      of thermal aggregation of rabbit muscle glyceraldehyde-3-phosphate dehydrogenase.
PG  - 11-25
LID - 10.1007/s10930-009-9217-9 [doi]
AB  - Effects of alpha-crystallin and GroEL on the kinetics of thermal aggregation of 
      rabbit muscle glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been studied 
      using dynamic light scattering and analytical ultracentrifugation. The analysis 
      of the initial parts of the dependences of the hydrodynamic radius of protein 
      aggregates on time shows that in the presence of alpha-crystallin or GroEL the 
      kinetic regime of GAPDH aggregation is changed from the regime of 
      diffusion-limited cluster-cluster aggregation to the regime of reaction-limited 
      cluster-cluster aggregation, wherein the sticking probability for the colliding 
      particles becomes lower the unity. In contrast to alpha-crystallin, GroEL does 
      not interfere with formation of the start aggregates which include denatured 
      GAPDH molecules. On the basis of the analytical ultracentrifugation data the 
      conclusion has been made that the products of dissociation of GAPDH and 
      alpha-crystallin or GroEL play an important role in the interactions of GAPDH and 
      chaperones at elevated temperatures.
FAU - Markossian, Kira A
AU  - Markossian KA
AD  - Bach Institute of Biochemistry, Russian Academy of Sciences, 119071, Moscow, 
      Russia. markossian@inbi.ras.ru
FAU - Golub, Nikolay V
AU  - Golub NV
FAU - Chebotareva, Natalia A
AU  - Chebotareva NA
FAU - Asryants, Regina A
AU  - Asryants RA
FAU - Naletova, Irina N
AU  - Naletova IN
FAU - Muronetz, Vladimir I
AU  - Muronetz VI
FAU - Muranov, Konstantin O
AU  - Muranov KO
FAU - Kurganov, Boris I
AU  - Kurganov BI
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Protein J
JT  - The protein journal
JID - 101212092
RN  - 0 (Escherichia coli Proteins)
RN  - 0 (GroE protein, E coli)
RN  - 0 (Heat-Shock Proteins)
RN  - 0 (alpha-Crystallins)
RN  - EC 1.2.1.- (Glyceraldehyde-3-Phosphate Dehydrogenases)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Escherichia coli Proteins/*chemistry
MH  - Glyceraldehyde-3-Phosphate Dehydrogenases/*chemistry
MH  - Heat-Shock Proteins/*chemistry
MH  - Kinetics
MH  - Male
MH  - Muscle, Skeletal/chemistry/*enzymology
MH  - Protein Conformation
MH  - Rabbits
MH  - Temperature
MH  - alpha-Crystallins/*chemistry
EDAT- 2009/11/26 06:00
MHDA- 2010/03/06 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/03/06 06:00 [medline]
AID - 10.1007/s10930-009-9217-9 [doi]
PST - ppublish
SO  - Protein J. 2010 Jan;29(1):11-25. doi: 10.1007/s10930-009-9217-9.