PMID- 19937267
OWN - NLM
STAT- MEDLINE
DCOM- 20100706
LR  - 20211020
IS  - 1572-9729 (Electronic)
IS  - 0923-9820 (Print)
IS  - 0923-9820 (Linking)
VI  - 21
IP  - 3
DP  - 2010 Jun
TI  - A beta-barrel outer membrane protein facilitates cellular uptake of 
      polychlorophenols in Cupriavidus necator.
PG  - 431-9
LID - 10.1007/s10532-009-9313-8 [doi]
AB  - The tcpRXABCYD operon of Cupriavidus necator JMP134 is involved in the 
      degradation of 2,4,6-trichlorophenol (TCP). All of the gene products except TcpY 
      have assigned functions in TCP metabolism. Sequence comparison identified TcpY as 
      a member of COG4313, a group of hypothetical proteins. TcpY has a signal peptide, 
      indicating it is a membrane or secreted protein. Secondary structure and topology 
      analysis indicated TcpY as a beta-barrel outer membrane protein, similar to the 
      Escherichia coli outer membrane protein FadL that transports hydrophobic 
      long-chain fatty acids. Constitutive expression of tcpY in two C. necator strains 
      rendered the cells more sensitive to TCP and other polychlorophenols. Further, C. 
      necator JMP134 expressing cloned tcpY transported more TCP into the cell than a 
      control with the cloning vector. Thus, TcpY is an outer membrane protein that 
      facilitates the passing of polychlorophenols across the outer membrane of C. 
      necator. Similarly, other COG4313 proteins are possibly outer membrane 
      transporters of hydrophobic aromatic compounds.
FAU - Belchik, Sara Mae
AU  - Belchik SM
AD  - School of Molecular Biosciences, Washington State University, Life Sciences 
      Building, Room 202, 100 Dairy Road, Pullman, WA 99164-7520, USA.
FAU - Schaeffer, Scott M
AU  - Schaeffer SM
FAU - Hasenoehrl, Shelley
AU  - Hasenoehrl S
FAU - Xun, Luying
AU  - Xun L
LA  - eng
GR  - T32 GM008336-19/GM/NIGMS NIH HHS/United States
GR  - T32 GM008336-20/GM/NIGMS NIH HHS/United States
GR  - T32 GM008336-21/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20091124
PL  - Netherlands
TA  - Biodegradation
JT  - Biodegradation
JID - 9100834
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Chlorophenols)
RN  - 0 (Disinfectants)
RN  - 0 (Environmental Pollutants)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
SB  - IM
MH  - Bacterial Outer Membrane Proteins/chemistry/genetics/*metabolism
MH  - Biodegradation, Environmental
MH  - Chlorophenols/*metabolism
MH  - Cupriavidus necator/genetics/*metabolism
MH  - Disinfectants/*metabolism
MH  - Environmental Pollutants/*metabolism
MH  - Gene Knockout Techniques
MH  - Phylogeny
MH  - Sequence Analysis, Protein
PMC - PMC2908400
MID - NIHMS217993
EDAT- 2009/11/26 06:00
MHDA- 2010/07/07 06:00
PMCR- 2010/07/22
CRDT- 2009/11/26 06:00
PHST- 2009/08/17 00:00 [received]
PHST- 2009/11/05 00:00 [accepted]
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/07/07 06:00 [medline]
PHST- 2010/07/22 00:00 [pmc-release]
AID - 10.1007/s10532-009-9313-8 [doi]
PST - ppublish
SO  - Biodegradation. 2010 Jun;21(3):431-9. doi: 10.1007/s10532-009-9313-8. Epub 2009 
      Nov 24.