PMID- 19937912
OWN - NLM
STAT- MEDLINE
DCOM- 20100811
LR  - 20181201
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 4
IP  - 2
DP  - 2010 Feb
TI  - Dynamic cell-cell interactions between cord blood haematopoietic progenitors and 
      the cellular niche are essential for the expansion of CD34+, CD34+CD38- and early 
      lymphoid CD7+ cells.
PG  - 149-58
LID - 10.1002/term.226 [doi]
AB  - Most clinical applications of haematopoietic stem/progenitor cells (HSCs) would 
      benefit from their ex vivo expansion to obtain a therapeutically significant 
      amount of cells from the available donor samples. We studied the impact of 
      cellular interactions between umbilical cord blood (UCB) haematopoietic cells and 
      bone marrow (BM)-derived mesenchymal stem cells (MSCs) on the ex vivo expansion 
      and differentiative potential of UCB CD34(+)-enriched cells. UCB cells were 
      cultured: (a) directly in contact with BM MSC-derived stromal layers (contact); 
      (b) separated by a microporous membrane (non-contact); or (c) without stroma (no 
      stroma). Highly dynamic culture events occurred in HSC-MSC co-cultures, involving 
      cell-cell interactions, which preceded HSC expansion. Throughout the time in 
      culture [18 days], total cell expansion was significantly higher in contact (fold 
      increase of 280 + or - 37 at day 18) compared to non-contact (85 + or - 25). No 
      significant cell expansion was observed in stroma-free cultures. CD34(+) cell 
      expansion was also clearly favoured by direct contact with BM MSCs (35 + or - 5- 
      and 7 + or - 3-fold increases at day 18 for contact and non-contact, 
      respectively). Moreover, a higher percentage of CD34(+)CD38(-) cells was 
      consistently maintained during the time in culture under contact (8.1 + or - 1.9% 
      at day 18) compared to non-contact (5.7 + or - 1.6%). Importantly, direct cell 
      interaction with BM MSCs significantly enhanced the expansion of early lymphoid 
      CD7(+) cells, yielding considerably higher (x3-10) progenitor numbers compared to 
      non-contact conditions. These results highlight the importance of dynamic 
      cell-cell interactions between UCB HSCs and BM MSCs, towards the maximization of 
      HSC expansion ex vivo to obtain clinically relevant cell numbers for multiple 
      settings, such as BM transplantation or somatic cell gene therapy.
FAU - da Silva, Claudia Lobato
AU  - da Silva CL
AD  - IBB-Institute for Biotechnology and Bioengineering, Centre for Biological and 
      Chemical Engineering, Instituto Superior Tecnico, Avenida Rovisco Pais, 1049-001 
      Lisbon, Portugal. claudia lobato@ist.utl.pt
FAU - Goncalves, Raquel
AU  - Goncalves R
FAU - dos Santos, Francisco
AU  - dos Santos F
FAU - Andrade, Pedro Z
AU  - Andrade PZ
FAU - Almeida-Porada, Graca
AU  - Almeida-Porada G
FAU - Cabral, Joaquim M S
AU  - Cabral JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Antigens, CD34)
RN  - 0 (Antigens, CD7)
RN  - 0 (Suspensions)
RN  - EC 3.2.2.6 (ADP-ribosyl Cyclase 1)
SB  - IM
MH  - ADP-ribosyl Cyclase 1/*metabolism
MH  - Antigens, CD34/*metabolism
MH  - Antigens, CD7/*metabolism
MH  - Cell Adhesion
MH  - *Cell Communication
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Colony-Forming Units Assay
MH  - Fetal Blood/*cytology
MH  - Flow Cytometry
MH  - Hematopoietic Stem Cells/*cytology/metabolism
MH  - Humans
MH  - Lymphocytes/cytology/metabolism
MH  - Mesenchymal Stem Cells/cytology/metabolism
MH  - Microscopy, Electron, Scanning
MH  - Phenotype
MH  - Stem Cell Niche/*cytology
MH  - Suspensions
EDAT- 2009/11/26 06:00
MHDA- 2010/08/12 06:00
CRDT- 2009/11/26 06:00
PHST- 2009/11/26 06:00 [entrez]
PHST- 2009/11/26 06:00 [pubmed]
PHST- 2010/08/12 06:00 [medline]
AID - 10.1002/term.226 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2010 Feb;4(2):149-58. doi: 10.1002/term.226.