PMID- 19938492
OWN - NLM
STAT- MEDLINE
DCOM- 20101005
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 27
IP  - 4
DP  - 2009 Jul
TI  - [Determination of residues of cyromazine and its metabolite melamine in chickens 
      by gas chromatography-mass spectrometry].
PG  - 401-5
AB  - A gas chromatography-mass spectrometric (GC-MS) method has been established for 
      the determination of cyromazine and its metabolite melamine in chickens. The 
      homogenized tissue samples added with melamine-15N3 were extracted with acidic 
      acetonitrile-water solution, and defatted with dichloromethane. The samples were 
      derivatized with N,O-bis(trimethylsilyl) trifluoroacetamide (BSTFA), after 
      solid-phase extraction (SPE) which was performed on Oasis MCX cartridges, and 
      then detected by GC-MS. Cyromazine was quantified by an external standard method, 
      and melamine by an internal standard method using melamine-15N3. The results 
      indicated that the linearities of cyromazine and melamine were within the range 
      of 100 - 1,000 microg/L, and their limits of quantification (LOQ) were 20 
      microg/kg. The mean recoveries of chicken samples fortified at three 
      concentrations of 20, 40 and 80 microg/kg were within the range of 75% - 110%. 
      The relative standard deviations (RSDs) of intra- and inter- batch were less than 
      10% and 15%, respectively. The application of this method was further 
      demonstrated by analyzing 10 real samples which were brought from local markets. 
      The results show that this method is simple, rapid, sensitive and specific. It is 
      appropriate for the identification and quantification of cyromazine and its 
      metabolite melamine in chickens.
FAU - Zhu, Xinle
AU  - Zhu X
AD  - China Institute of Veterinary Drug Control, Beijing, China. zhuxinle@ivdc.gov.cn
FAU - Liu, Qi
AU  - Liu Q
FAU - Li, Dan
AU  - Li D
FAU - Wang, Shuhuai
AU  - Wang S
FAU - Wang, Xia
AU  - Wang X
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Pesticide Residues)
RN  - 0 (Triazines)
RN  - CA49Y29RA9 (cyromazine)
RN  - N3GP2YSD88 (melamine)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Food Contamination/analysis
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Meat/*analysis
MH  - Pesticide Residues/*analysis
MH  - Triazines/*analysis
EDAT- 2009/11/27 06:00
MHDA- 2010/10/06 06:00
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/10/06 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2009 Jul;27(4):401-5.