PMID- 19938494
OWN - NLM
STAT- MEDLINE
DCOM- 20101005
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 27
IP  - 4
DP  - 2009 Jul
TI  - [Simultaneous determination of tetracyclines, sulfonamides and quinolones 
      residues in chicken livers by ultra performance liquid chromatography-tandem mass 
      spectrometry].
PG  - 412-6
AB  - An analytical method for the simultaneous determination of 3 tetracyclines, 10 
      sulfonamides and 8 quinolones in chicken livers by ultra performance liquid 
      chromatography coupled with tandem quadrupole mass spectrometry (UPLC-MS/MS) in 
      positive ion mode with multiple reaction monitoring (MRM) has been developed and 
      validated. A total of 2 g homogenized sample of chicken livers was placed in a 50 
      mL polypropylene tube, and 1 mL of McIlvaine buffer and 4 mL of acetonitrile were 
      added. After stirring and centrifuging for 5 min at 4,000 r/min, the supernatant 
      was collected and the remains was extracted by 5 mL acetonitrile. The supernatant 
      was merged together and evaporated to dryness under a steam of nitrogen at 60 
      degrees C. The residue was dissolved with 4 mL of phosphoric acid-triethylamine 
      buffer-acetonitrile (85:15, v/v) and 4 mL of n-hexane. After stirring for 1 min 
      and centrifuging for 5 min at 4 000 r/min, the under layer solution was analyzed 
      using UPLC-MS/MS. The satisfactory recoveries (66.8% - 128.5%) of all the 
      veterinary drugs were demonstrated at spiked levels of 5, 10 and 50 microg/kg 
      with the overall relative standard deviations (RSDs) for intra-day and inter-day 
      of the 21 analytes less than 20.5%. The limit of detection (LOD) and the limit of 
      quantification (LOQ) were 2 microg/kg and 5 microg/kg, respectively for each 
      drug. This method has good stability, lower detection limits and can be used as a 
      conclusive evidence method of these drug residues in chicken livers.
FAU - Guo, Liming
AU  - Guo L
AD  - College of Veterinary Medicine, China Agricultural University, Beijing, China.
FAU - Zhu, Kui
AU  - Zhu K
FAU - Jiang, Haiyang
AU  - Jiang H
FAU - Li, Jiancheng
AU  - Li J
FAU - Li, Xiaowei
AU  - Li X
FAU - Ding, Shuangyang
AU  - Ding S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Quinolones)
RN  - 0 (Sulfonamides)
RN  - 0 (Tetracyclines)
SB  - IM
MH  - Animals
MH  - Chickens
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Residues/analysis
MH  - Food Contamination/analysis
MH  - Liver/*chemistry
MH  - Quinolones/*analysis
MH  - Sulfonamides/*analysis
MH  - Tandem Mass Spectrometry/methods
MH  - Tetracyclines/*analysis
EDAT- 2009/11/27 06:00
MHDA- 2010/10/06 06:00
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/10/06 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2009 Jul;27(4):412-6.