PMID- 19938495
OWN - NLM
STAT- MEDLINE
DCOM- 20101005
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 27
IP  - 4
DP  - 2009 Jul
TI  - [Determination of 9 cephalosporin drug residues in beef by ultra performance 
      liquid chromatography-tandem mass spectrometry].
PG  - 417-20
AB  - A confirmative method to determine 9 cephalosporin residues in beef by ultra 
      performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) was 
      developed. The sample was homogenized and extracted with acetonitrile and water 
      for 1 min at 14,000 r/min, centrifuged at 10,000 r/min and 4 degrees C for 10 
      min. A total of 2 mL saturated sodium chloride solution was added to avoid 
      foaming during the acetonitrile evaporation, the acetonitrile was evaporated 
      below 37 degrees C using a rotary evaporator, and then cleaned up on an Oasis HLB 
      (500 mg, 6 mL) SPE column by washing with 5 mL water and eluting with 
      acetonitrile-water (7:3, v/v). The eluate was blown to dryness under a stream of 
      nitrogen and adjusted to 3.0 mL with water. The separation was carried out on an 
      ACQUITY UPLC BEH C18 column within 5 min, analyzed by UPLC-MS/MS system with 
      external standard method. The limits of quantification (LOQs) of cefuroxime, 
      ceftiofur and cefalonium were 10, 0.5 and 0.5 microg/kg, respectively; the LOQs 
      of other cephalosporins were 1.0 microg/kg. The recoveries of cephalosporins 
      ranged from 74.2% to 119% and the relative standard deviations (RSDs) ranged from 
      2.9% to 15% for the spiked beef sample. The method is quick, easy, very sensitive 
      and suitable for the determination of cephalosporin residues in beef.
FAU - Bai, Guotao
AU  - Bai G
AD  - Inner Mongolia Entry-Exit Inspection and Quarantine Bureau, Hohhot, China.
FAU - Chu, Xiaogang
AU  - Chu X
FAU - Pan, Guoqing
AU  - Pan G
FAU - Li, Xiuqin
AU  - Li X
FAU - Yong, Wei
AU  - Yong W
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Cephalosporins)
RN  - 83JL932I1C (ceftiofur)
RN  - K3086GQJ9Z (cefaloram)
RN  - O1R9FJ93ED (Cefuroxime)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cefuroxime/analysis
MH  - Cephalosporins/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Drug Residues/*analysis
MH  - Food Contamination/analysis
MH  - Meat/*analysis
MH  - Tandem Mass Spectrometry/*methods
EDAT- 2009/11/27 06:00
MHDA- 2010/10/06 06:00
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/10/06 06:00 [medline]
PST - ppublish
SO  - Se Pu. 2009 Jul;27(4):417-20.