PMID- 19939462
OWN - NLM
STAT- MEDLINE
DCOM- 20100628
LR  - 20211020
IS  - 1873-2534 (Electronic)
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 135
IP  - 1-2
DP  - 2010 May 15
TI  - Characterization of the cytokine and maturation responses of pure populations of 
      porcine plasmacytoid dendritic cells to porcine viruses and toll-like receptor 
      agonists.
PG  - 20-33
LID - S0165-2427(09)00352-3 [pii]
LID - 10.1016/j.vetimm.2009.10.026 [doi]
AB  - Plausible representatives of plasmacytoid dendritic cells (pDCs) in pigs have 
      been characterized as being CD4(hi)CD172(lo). Due to their paucity in blood, we 
      utilized novel fluorescent-activated cell sorting procedures to isolate them from 
      PBMC. The resultant subset was greater than 98% homogeneous in regards to the 
      selected phenotype and contained the preponderance of individuals secreting 
      IFN-alpha after exposure to a known stimulant, transmissible gastroenteritis 
      virus (TGEV). In addition to being a potent source of IFN-alpha, other properties 
      of these porcine CD4(hi)CD172(lo) cells including their morphological transition 
      from a plasma cell-like shape during quiescence to one resembling a dendritic 
      cell (DC) after activation by TGEV and their relatively strong constitutive 
      expression of interferon regulatory factor-7 (IRF-7) conformed to the 
      expectations of genuine pDCs. While a substantial IFN-alpha response was also 
      elicited from the porcine pDCs by pseudorabies virus (PrV), swine influenza virus 
      (SIV), and TLR7 and 9 agonists, there was an agent-dependent induction of varying 
      amounts of IL-2, IL-6, IL-8, IL-12, IFN-gamma, and TNF-alpha. Notably, porcine 
      reproductive and respiratory syndrome virus (PRRSV) failed to provoke the pDCs to 
      secrete any of the measured cytokines except IL-2. Moreover, whereas pDCs exposed 
      to TGEV or the TLR9 agonist rapidly increased IRF-7 production and morphed into 
      DCs with enhanced CD80/86 expression, similar alterations were not observed 
      during incubation with PRRSV. This atypical response of pDCs to PRRSV may 
      contribute to its pathogenesis, which unlike that associated with PrV, SIV or 
      TGEV includes persistent infection and limited development of protective 
      immunity.
CI  - Copyright 2009 Elsevier B.V. All rights reserved.
FAU - Calzada-Nova, Gabriela
AU  - Calzada-Nova G
AD  - Department of Pathobiology, College of Veterinary Medicine, University of 
      Illinois, 2001 South Lincoln Avenue, Urbana, IL, USA.
FAU - Schnitzlein, William
AU  - Schnitzlein W
AD  - Department of Pathobiology, College of Veterinary Medicine, University of 
      Illinois, 2001 South Lincoln Avenue, Urbana, IL, USA.
FAU - Husmann, Robert
AU  - Husmann R
AD  - Department of Pathobiology, College of Veterinary Medicine, University of 
      Illinois, 2001 South Lincoln Avenue, Urbana, IL, USA.
FAU - Zuckermann, Federico A
AU  - Zuckermann FA
AD  - Department of Pathobiology, College of Veterinary Medicine, University of 
      Illinois, 2001 South Lincoln Avenue, Urbana, IL, USA. Electronic address: 
      fazaaa@illinois.edu.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20091030
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Aminoquinolines)
RN  - 0 (Cytokines)
RN  - 0 (Interferon Regulatory Factor-7)
RN  - 0 (Interferon-alpha)
RN  - 0 (Toll-Like Receptors)
RN  - P1QW714R7M (Imiquimod)
SB  - IM
MH  - Aminoquinolines/pharmacology
MH  - Animals
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - Cytokines/biosynthesis/*immunology
MH  - Dendritic Cells/drug effects/*immunology/virology
MH  - Flow Cytometry/veterinary
MH  - Gastroenteritis, Transmissible, of Swine/immunology
MH  - Herpesvirus 1, Suid/immunology
MH  - Imiquimod
MH  - Interferon Regulatory Factor-7/immunology
MH  - Interferon-alpha/immunology
MH  - Orthomyxoviridae/immunology
MH  - Orthomyxoviridae Infections/immunology
MH  - Porcine Reproductive and Respiratory Syndrome/immunology
MH  - Porcine respiratory and reproductive syndrome virus/immunology
MH  - Pseudorabies/immunology
MH  - Swine/*immunology/virology
MH  - Swine Diseases/immunology/virology
MH  - Toll-Like Receptors/*agonists/immunology
MH  - Transmissible gastroenteritis virus/immunology
PMC - PMC7126865
EDAT- 2009/11/27 06:00
MHDA- 2010/06/29 06:00
PMCR- 2009/10/30
CRDT- 2009/11/27 06:00
PHST- 2009/07/10 00:00 [received]
PHST- 2009/10/13 00:00 [revised]
PHST- 2009/10/21 00:00 [accepted]
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/06/29 06:00 [medline]
PHST- 2009/10/30 00:00 [pmc-release]
AID - S0165-2427(09)00352-3 [pii]
AID - 10.1016/j.vetimm.2009.10.026 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2010 May 15;135(1-2):20-33. doi: 
      10.1016/j.vetimm.2009.10.026. Epub 2009 Oct 30.