PMID- 19939971
OWN - NLM
STAT- MEDLINE
DCOM- 20100217
LR  - 20200930
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Linking)
VI  - 298
IP  - 2
DP  - 2010 Feb
TI  - Gene expression profiling of the short-term adaptive response to acute caloric 
      restriction in liver and adipose tissues of pigs differing in feed efficiency.
PG  - R494-507
LID - 10.1152/ajpregu.00632.2009 [doi]
AB  - Residual feed intake (RFI) is a measure of feed efficiency, in which low RFI 
      denotes improved feed efficiency. Caloric restriction (CR) is associated with 
      feed efficiency in livestock species and to human health benefits, such as 
      longevity and cancer prevention. We have developed pig lines that differ in RFI, 
      and we are interested in identifying the genes and pathways that underlie feed 
      efficiency. Prepubertal Yorkshire gilts with low RFI (n = 10) or high RFI (n = 
      10) were fed ad libitum or fed at restricted intake of 80% of maintenance energy 
      requirements for 8 days. We measured serum metabolites and hormones and generated 
      transcriptional profiles of liver and subcutaneous adipose tissue on these 
      animals. Overall, 6,114 genes in fat and 305 genes in liver were differentially 
      expressed (DE) in response to CR, and 311 genes in fat and 147 genes in liver 
      were DE due to RFI differences. Pathway analyses of CR-induced DE genes indicated 
      a dramatic switch to a conservation mode of energy usage by down-regulating 
      lipogenesis and steroidogenesis in both liver and fat. Interestingly, CR altered 
      expression of genes in immune and cell cycle/apoptotic pathways in fat, which may 
      explain part of the CR-driven lifespan enhancement. In silico analysis of 
      transcription factors revealed ESR1 as a putative regulator of the adaptive 
      response to CR, as several targets of ESR1 in our DE fat genes were annotated as 
      cell cycle/apoptosis genes. The lipid metabolic pathway was overrepresented by 
      down-regulated genes due to both CR and low RFI. We propose a common energy 
      conservation mechanism, which may be controlled by PPARA, PPARG, and/or CREB in 
      both CR and feed-efficient pigs.
FAU - Lkhagvadorj, Sender
AU  - Lkhagvadorj S
AD  - Department of Animal Science, Iowa State University, 2255 Kildee Hall, Ames, IA 
      50011-3150, USA.
FAU - Qu, Long
AU  - Qu L
FAU - Cai, Weiguo
AU  - Cai W
FAU - Couture, Oliver P
AU  - Couture OP
FAU - Barb, C Richard
AU  - Barb CR
FAU - Hausman, Gary J
AU  - Hausman GJ
FAU - Nettleton, Dan
AU  - Nettleton D
FAU - Anderson, Lloyd L
AU  - Anderson LL
FAU - Dekkers, Jack C M
AU  - Dekkers JC
FAU - Tuggle, Christopher K
AU  - Tuggle CK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20091125
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 63231-63-0 (RNA)
SB  - IM
MH  - Adaptation, Physiological/*physiology
MH  - Adipose Tissue/*physiology
MH  - Adiposity/physiology
MH  - Animal Feed
MH  - Animals
MH  - Body Weight/physiology
MH  - *Caloric Restriction
MH  - Eating
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Liver/*physiology
MH  - Longevity/genetics/physiology
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA/biosynthesis/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Species Specificity
MH  - Swine
MH  - Transcription, Genetic/physiology
MH  - Weight Gain/physiology
EDAT- 2009/11/27 06:00
MHDA- 2010/02/18 06:00
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/02/18 06:00 [medline]
AID - 00632.2009 [pii]
AID - 10.1152/ajpregu.00632.2009 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2010 Feb;298(2):R494-507. doi: 
      10.1152/ajpregu.00632.2009. Epub 2009 Nov 25.