PMID- 19940123
OWN - NLM
STAT- MEDLINE
DCOM- 20100222
LR  - 20211020
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 285
IP  - 4
DP  - 2010 Jan 22
TI  - Nuclear factor 1 synergizes with progesterone receptor on the mouse mammary tumor 
      virus promoter wrapped around a histone H3/H4 tetramer by facilitating access to 
      the central hormone-responsive elements.
PG  - 2622-31
LID - 10.1074/jbc.M109.060848 [doi]
AB  - Steroid hormones induce transcription of their responsive genes by complex 
      mechanisms including synergism between the hormone receptors and other 
      transcription factors. On the mouse mammary tumor virus (MMTV) promoter 
      progesterone induction is mediated by the reciprocal synergism between 
      progesterone receptor (PR) and the ubiquitous transcription factor nuclear factor 
      1 (NF1). PR binding mediates ATP-dependent displacement of histone H2A and H2B, 
      enabling NF1 access to its target site. In minichromosomes assembled in vitro NF1 
      binding facilitates access of PR to the hormone-responsive elements (HREs) by 
      precluding reforming of the histone octamer, but the function of NF1 in living 
      cells remains unclear. Here we show that depleting NF1 by small interfering RNAs 
      or mutating the NF1-binding site significantly compromises transcription of the 
      MMTV promoter. The central HREs 2 and 3 are not needed for ATP-dependent H2A/H2B 
      displacement or NF1 binding but are critical for full PR binding and MMTV 
      transactivation. We found that NF1 binding to the MMTV promoter on a H3/H4 
      histone tetramer particle exposes the central HREs and facilitates their binding 
      by PR, suggesting a possible mechanism for the reciprocal synergism between PR 
      and NF1.
FAU - Vicent, Guillermo Pablo
AU  - Vicent GP
AD  - Centre de Regulacio Genomica, Universitat Pompeu Fabra, Parc de Recerca 
      Biomedica, Dr Aiguader 88, E-08003 Barcelona, Spain. guillermo.vicent@crg.es
FAU - Zaurin, Roser
AU  - Zaurin R
FAU - Nacht, A Silvina
AU  - Nacht AS
FAU - Font-Mateu, Jofre
AU  - Font-Mateu J
FAU - Le Dily, Francois
AU  - Le Dily F
FAU - Beato, Miguel
AU  - Beato M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091124
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Chromatin)
RN  - 0 (Histones)
RN  - 0 (Hormones)
RN  - 0 (NFI Transcription Factors)
RN  - 0 (Nucleosomes)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Receptors, Progesterone)
RN  - EC 1.13.12.- (Luciferases)
SB  - IM
MH  - Breast Neoplasms
MH  - Cell Line, Tumor
MH  - Chromatin/physiology
MH  - Female
MH  - Histones/*metabolism
MH  - Hormones/metabolism
MH  - Humans
MH  - Luciferases/genetics
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - NFI Transcription Factors/*metabolism
MH  - Nucleosomes/physiology
MH  - Promoter Regions, Genetic/physiology
MH  - RNA, Small Interfering
MH  - Receptors, Progesterone/*metabolism
MH  - Response Elements/physiology
MH  - Transcription, Genetic/physiology
MH  - Transcriptional Activation/physiology
PMC - PMC2807319
EDAT- 2009/11/27 06:00
MHDA- 2010/02/23 06:00
PMCR- 2011/01/22
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/02/23 06:00 [medline]
PHST- 2011/01/22 00:00 [pmc-release]
AID - S0021-9258(19)63756-7 [pii]
AID - M109.060848 [pii]
AID - 10.1074/jbc.M109.060848 [doi]
PST - ppublish
SO  - J Biol Chem. 2010 Jan 22;285(4):2622-31. doi: 10.1074/jbc.M109.060848. Epub 2009 
      Nov 24.