PMID- 19940396
OWN - NLM
STAT- MEDLINE
DCOM- 20100408
LR  - 20190606
IS  - 0916-7250 (Print)
IS  - 0916-7250 (Linking)
VI  - 72
IP  - 2
DP  - 2010 Feb
TI  - Cultivation and characterization of canine skin-derived mast cells.
PG  - 131-40
AB  - It is essential to develop a technique to culture purified skin-derived mast 
      cells (SMCs) to facilitate immunological research on allergic diseases in dogs. 
      This study was performed to develop an efficient culture system for canine SMCs 
      and to characterize the cells in comparison to canine bone marrow-derived mast 
      cells (BMMCs). Enzymatically digested skin biopsy samples were cultivated in 
      serum-free AIM-V medium supplemented with recombinant canine stem cell factor. 
      Three to five weeks after the initiation of culture, mast cells were collected by 
      a magnetic activated cell separation system using anti-c-Kit antibody. The 
      collected cells were composed of a uniform population showing morphological 
      characteristics of mast cells with a round or oval nucleus and abundant toluidine 
      blue-positive metachromatic granules in the cytoplasm. The results of flow 
      cytometric analysis for the presence of cell membrane c-Kit and Fc epsilon 
      receptor I (FcepsilonRI) indicated that approximately 90% of the cells were mast 
      cells. The cytoplasmic granules were positive for both tryptase and chymase. 
      Apparent dose-dependent degranulation was induced by antibody-mediated 
      cross-linking of immunoglobulin E (IgE) bound to the cells. These cytological and 
      immunological characteristics observed in SMCs were mostly similar to those 
      observed in BMMCs; however, IgE-mediated degranulation was significantly lower in 
      SMCs than BMMCs. The culture system for canine SMCs developed in this study would 
      be useful in understanding the pathophysiology and developing anti-allergic 
      therapeutics in canine allergic dermatitis.
FAU - Kawarai, Shinpei
AU  - Kawarai S
AD  - First Department of Veterinary Microbiology, School of Veterinary Medicine, Azabu 
      University, 1-7-71 Fuchinobe, Sagamihara, Kanagawa 229-8501, Japan.
FAU - Masuda, Kenichi
AU  - Masuda K
FAU - Ohmori, Keitaro
AU  - Ohmori K
FAU - Matsuura, Shinobu
AU  - Matsuura S
FAU - Yasuda, Nobutaka
AU  - Yasuda N
FAU - Nagata, Masahiko
AU  - Nagata M
FAU - Sakaguchi, Masahiro
AU  - Sakaguchi M
FAU - Tsujimoto, Hajime
AU  - Tsujimoto H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091125
PL  - Japan
TA  - J Vet Med Sci
JT  - The Journal of veterinary medical science
JID - 9105360
RN  - 0 (Azure Stains)
RN  - 0 (Benzidines)
RN  - 0 (Receptors, IgE)
RN  - 63HLO2IV6K (2-tolidine)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-kit)
RN  - EC 3.2.1.52 (beta-N-Acetylhexosaminidases)
RN  - EC 3.4.21.39 (Chymases)
RN  - EC 3.4.21.59 (Tryptases)
SB  - IM
MH  - Animals
MH  - Azure Stains/chemistry
MH  - Benzidines/chemistry
MH  - Cell Culture Techniques/methods/*veterinary
MH  - Chymases/analysis
MH  - Dermatitis, Allergic Contact/immunology/*veterinary
MH  - Dogs
MH  - Female
MH  - Flow Cytometry/veterinary
MH  - Immunohistochemistry/veterinary
MH  - Male
MH  - Mast Cells/cytology/enzymology/*immunology/ultrastructure
MH  - Microscopy, Electron, Transmission/veterinary
MH  - Proto-Oncogene Proteins c-kit/analysis
MH  - Receptors, IgE/analysis
MH  - Skin/cytology/*immunology/ultrastructure
MH  - Tryptases/analysis
MH  - beta-N-Acetylhexosaminidases/analysis
EDAT- 2009/11/27 06:00
MHDA- 2010/04/09 06:00
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/04/09 06:00 [medline]
AID - JST.JSTAGE/jvms/09-0281 [pii]
AID - 10.1292/jvms.09-0281 [doi]
PST - ppublish
SO  - J Vet Med Sci. 2010 Feb;72(2):131-40. doi: 10.1292/jvms.09-0281. Epub 2009 Nov 
      25.