PMID- 19941115
OWN - NLM
STAT- MEDLINE
DCOM- 20100202
LR  - 20201226
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 595
DP  - 2010
TI  - The isolation of mouse dendritic cells from lymphoid tissues and the 
      identification of dendritic cell subtypes by multiparameter flow cytometry.
PG  - 205-29
LID - 10.1007/978-1-60761-421-0_14 [doi]
AB  - Dendritic cells (DCs) are powerful initiators of the adaptive immune system and 
      their manipulation is seen as a viable approach in the treatment of many human 
      ailments. Our isolation method purifies DCs from mouse lymphoid organs by 
      efficiently removing them from the tissue using collagenase, selecting the light 
      density fraction of cells and then negatively selecting for DCs using a 
      combination of monoclonal antibodies directed against non-DC lineage cells 
      together with anti-rat immunoglobulin-coated magnetic beads. Remaining 
      contaminating cells can be depleted using a combination of specific biotinylated 
      antibodies and anti-biotin-coated magnetic beads, in the case of natural killer 
      (NK) cells, or by FACS gating, during sorting or analysis, in the case of 
      autofluorescent macrophages. The heterogeneous DCs can then be separated into 
      various functionally different populations via immunofluorescent labelling and 
      cell sorting. The study of mouse DC populations may enable us to align them to 
      their human counterparts and subsequently to DC populations produced in vitro.
FAU - Vremec, David
AU  - Vremec D
AD  - The Walter and Eliza Hall Institute of Medical Research, Parkville, VIC, 
      Australia.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Antigens, CD)
RN  - 0 (CD11b Antigen)
RN  - 0 (CD24 Antigen)
RN  - 0 (CD8 Antigens)
RN  - 0 (DEC-205 receptor)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antigens, CD/metabolism
MH  - CD11b Antigen/metabolism
MH  - CD24 Antigen/metabolism
MH  - CD8 Antigens/metabolism
MH  - Dendritic Cells/*cytology/metabolism
MH  - Flow Cytometry/*methods
MH  - Humans
MH  - Immunomagnetic Separation/methods
MH  - Killer Cells, Natural/cytology/metabolism
MH  - Lectins, C-Type/metabolism
MH  - Lymphoid Tissue/*cytology
MH  - Mice
MH  - Minor Histocompatibility Antigens
MH  - Rats
MH  - Receptors, Cell Surface/metabolism
EDAT- 2009/11/27 06:00
MHDA- 2010/02/03 06:00
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/02/03 06:00 [medline]
AID - 10.1007/978-1-60761-421-0_14 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;595:205-29. doi: 10.1007/978-1-60761-421-0_14.