PMID- 19941123
OWN - NLM
STAT- MEDLINE
DCOM- 20100202
LR  - 20091126
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 595
DP  - 2010
TI  - Studying the function of dendritic cells in mouse models of asthma.
PG  - 331-49
LID - 10.1007/978-1-60761-421-0_22 [doi]
AB  - Dendritic cells (DCs) are known to play a crucial role in the induction of 
      allergic asthma in mouse models. Their antigen presentation capacity, linked to 
      their capacity to prime naive T cells and polarize them towards a Th1, Th2, Th17 
      or Treg profile, allows them to efficiently initiate an immune response to 
      allergens. Airway dendritic cells also play a crucial role in the local 
      restimulation of circulating effector T cells upon allergen challenge. Given 
      their important implication in pathogenesis of asthma in mice models, the study 
      of environmental and pharmacologic effects on DCs function is now a blooming 
      field. There is therefore a critical need for a stable, yet flexible animal model 
      to investigate the effects of various environmental factors (endotoxins, 
      pollutants, etc.) or pharmacologic molecules on DCs and subsequently on their 
      role in asthma pathogenesis. This chapter presents an approach using a reliable 
      animal model of asthma that has the advantage to allow interventions on DCs 
      before their use to induce allergic asthma. We also cover some of the endpoint 
      techniques used to assess asthma and the immune reactions involved in its 
      pathogenesis.
FAU - Pouliot, Philippe
AU  - Pouliot P
AD  - Laboratory of Immunoregulation and Mucosal Immunology, Department of Pulmonary 
      Medicine, Gent University, Gent, Belgium.
FAU - Willart, Monique A
AU  - Willart MA
FAU - Hammad, Hamida
AU  - Hammad H
FAU - Lambrecht, Bart N
AU  - Lambrecht BN
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Asthma/*immunology
MH  - Bone Marrow Cells/cytology
MH  - Dendritic Cells/*cytology/*microbiology
MH  - Disease Models, Animal
MH  - Flow Cytometry
MH  - Immunophenotyping
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
EDAT- 2009/11/27 06:00
MHDA- 2010/02/03 06:00
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/02/03 06:00 [medline]
AID - 10.1007/978-1-60761-421-0_22 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;595:331-49. doi: 10.1007/978-1-60761-421-0_22.