PMID- 19941124
OWN - NLM
STAT- MEDLINE
DCOM- 20100202
LR  - 20091126
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 595
DP  - 2010
TI  - Direct ex vivo activation of T cells for analysis of dendritic cells antigen 
      presentation.
PG  - 351-69
LID - 10.1007/978-1-60761-421-0_23 [doi]
AB  - Dendritic cells (DCs) are a heterogeneous population of professional 
      antigen-presenting cells (APCs) that play a major role in the initiation of 
      immune responses. DC subsets differ in their anatomical locations together with 
      their intrinsic abilities to capture, process, and present antigens on their 
      major histocompatibility (MHC) class I and class II molecules. These features 
      enable each DC subset to have distinct roles in immunity to infection and in the 
      maintenance of self-tolerance. The discrete features of DC subpopulations have 
      largely been defined by cell surface phenotype and anatomical location, rather 
      than function. We have developed direct ex vivo methods to efficiently isolate 
      small numbers of DCs from lymph node (LN) draining tissues and infectious sites 
      to allow fine probing of their function using very sensitive antigen-specific 
      LacZ hybridomas and in vitro proliferation of CFSE-labeled T cells. These 
      approaches are particularly sensitive for detecting endogenous antigens derived 
      from pathogens and self-tissues. Understanding these interactions has begun to 
      allow us to understand how integration of different populations in the DC network 
      responds to multiple scenarios of infection.
FAU - Belz, Gabrielle T
AU  - Belz GT
AD  - The Walter and Eliza Hall Institute of Medical Research, Parkville, Australia.
LA  - eng
GR  - Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Antigen Presentation/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Lymphocyte Activation/*physiology
MH  - Mice
MH  - T-Lymphocytes/*immunology
EDAT- 2009/11/27 06:00
MHDA- 2010/02/03 06:00
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/02/03 06:00 [medline]
AID - 10.1007/978-1-60761-421-0_23 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;595:351-69. doi: 10.1007/978-1-60761-421-0_23.