PMID- 19941128
OWN - NLM
STAT- MEDLINE
DCOM- 20100202
LR  - 20091126
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 595
DP  - 2010
TI  - Generation of parabiotic mice for the study of DC and DC precursor circulation.
PG  - 413-28
LID - 10.1007/978-1-60761-421-0_27 [doi]
AB  - Dendritic cells (DCs) are the main regulators of adaptive immune responses and 
      considerable interest currently focuses on the mechanisms of DC homeostasis. 
      Understanding the mechanisms and regulation of DC generation may provide cues on 
      how to modulate DC numbers and their longevity, an issue of wide interest in 
      translational research. Surgical joining of the blood circulation of two mice 
      (parabiosis) results in the equal distribution of lymphocytes between the 
      parabiotic partners after a short period of time. In contrast, DCs fail to 
      equalize even after prolonged periods of parabiosis suggesting that self-renewing 
      precursors replenish mature DCs in situ. We have shown that mature DCs are 
      constantly replenished by blood-borne precursors. Furthermore, the low but 
      sustained exchange rate of DCs during parabiosis depends on fms-like tyrosine 
      kinase 3 (Flt3)-mediated signals, which are important regulators for maintenance 
      of normal DC numbers in the spleen by controlling their division in situ.
FAU - Waskow, Claudia
AU  - Waskow C
AD  - CRTD/DFG Center for Regenerative Therapies, TU Dresden, Dresden, Germany.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - EC 2.7.10.1 (fms-Like Tyrosine Kinase 3)
SB  - IM
MH  - Animals
MH  - Dendritic Cells/*cytology
MH  - Flow Cytometry
MH  - Hemolysis
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Mutant Strains
MH  - Parabiosis/*methods
MH  - Signal Transduction/physiology
MH  - fms-Like Tyrosine Kinase 3/metabolism
EDAT- 2009/11/27 06:00
MHDA- 2010/02/03 06:00
CRDT- 2009/11/27 06:00
PHST- 2009/11/27 06:00 [entrez]
PHST- 2009/11/27 06:00 [pubmed]
PHST- 2010/02/03 06:00 [medline]
AID - 10.1007/978-1-60761-421-0_27 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;595:413-28. doi: 10.1007/978-1-60761-421-0_27.