PMID- 19944210
OWN - NLM
STAT- MEDLINE
DCOM- 20100203
LR  - 20131121
IS  - 1531-5037 (Electronic)
IS  - 0022-3468 (Linking)
VI  - 44
IP  - 11
DP  - 2009 Nov
TI  - Effects of the administration of pentoxifylline and prednisolone on the evolution 
      of portal fibrogenesis secondary to biliary obstruction-an experimental study in 
      growing animals.
PG  - 2071-7
LID - 10.1016/j.jpedsurg.2009.05.020 [doi]
AB  - BACKGROUND: Many chronic liver diseases lead to progressive hepatic fibrosis, a 
      condition that can ultimately result in loss of organ function and severe portal 
      hypertension necessitating hepatic transplantation. Within the last few decades, 
      studies have been conducted to demonstrate the possibility of drug modulation of 
      hepatic fibrogenesis. Regarding biliary obstruction, it has been suggested that 
      administration of corticosteroids could promote better late outcomes for children 
      with biliary atresia submitted to Kasai's portoenterostomy. Models used to test 
      potential antifibrogenic drugs such as pentoxifylline (PTX) have not included 
      growing animals. METHODS: In this experimental study, 119 young rats (21st or 
      22nd days) were submitted to laparotomy and common bile duct ligation (CBDL) or 
      to sham surgery (SHAM). Animals were allocated into 5 groups, according to 
      surgical procedure, and administered the following solutions: (1) CBDL + 
      distilled water, (2) SHAM + distilled water, (3) CBDL + PTX, (4) CBDL + 
      prednisolone (PRED), and (5) CBDL + PTX + PRED (PTX + PRED). Each group was 
      further divided into 2 subgroups according to the length of the experiment (15 or 
      30 days). At the end of the defined period, animals were weighed, and a hepatic 
      fragment was collected from each one for analyses. RESULTS: The PTX animals 
      exhibited increased weight gain compared to animals in the PRED or PTX + PRED 
      groups. Animals from the 3 therapeutic groups (PTX, PRED, and PTX + PRED) showed 
      diminished collagen-filled area in portal spaces. Total portal space area was 
      increased in the PTX group. CONCLUSIONS: Hepatic fibrosis induced by bile duct 
      ligation in young rats could be modulated by pharmacologic interventions. 
      Administration of PTX or PRED, or the combination of both, resulted in diminished 
      collagen-filled areas in portal spaces.
FAU - Andrade, Wagner de Castro
AU  - Andrade Wde C
AD  - Pediatric Surgery Division, Laboratory of Pediatric Surgery, University of Sao 
      Paulo Medical School, CEP 01246-903 Sao Paulo, Brazil.
FAU - Tannuri, Uenis
AU  - Tannuri U
FAU - da Silva, Luiz Fernando Ferraz
AU  - da Silva LF
FAU - Alves, Venancio Avancini Ferreira
AU  - Alves VA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
RN  - 0 (Glucocorticoids)
RN  - 0 (Phosphodiesterase Inhibitors)
RN  - 9PHQ9Y1OLM (Prednisolone)
RN  - SD6QCT3TSU (Pentoxifylline)
SB  - IM
MH  - Animals
MH  - Biliary Atresia/complications/drug therapy
MH  - Biliary Tract Diseases/congenital
MH  - Cholestasis/complications/*prevention & control
MH  - Common Bile Duct Diseases/complications/prevention & control
MH  - Disease Models, Animal
MH  - Drug Therapy, Combination
MH  - Glucocorticoids/*pharmacology/therapeutic use
MH  - Humans
MH  - Hypertension, Portal/*drug therapy
MH  - Ligation
MH  - Liver Cirrhosis, Experimental/etiology/*prevention & control
MH  - Pentoxifylline/*pharmacology/therapeutic use
MH  - Phosphodiesterase Inhibitors/*pharmacology/therapeutic use
MH  - Prednisolone/*pharmacology/therapeutic use
MH  - Rats
MH  - Rats, Wistar
MH  - Weight Gain/drug effects
EDAT- 2009/12/01 06:00
MHDA- 2010/02/04 06:00
CRDT- 2009/12/01 06:00
PHST- 2009/02/24 00:00 [received]
PHST- 2009/05/10 00:00 [revised]
PHST- 2009/05/11 00:00 [accepted]
PHST- 2009/12/01 06:00 [entrez]
PHST- 2009/12/01 06:00 [pubmed]
PHST- 2010/02/04 06:00 [medline]
AID - S0022-3468(09)00414-X [pii]
AID - 10.1016/j.jpedsurg.2009.05.020 [doi]
PST - ppublish
SO  - J Pediatr Surg. 2009 Nov;44(11):2071-7. doi: 10.1016/j.jpedsurg.2009.05.020.