PMID- 19945493
OWN - NLM
STAT- MEDLINE
DCOM- 20100407
LR  - 20100201
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 71
IP  - 2
DP  - 2010 Feb
TI  - Computational characterization of Plasmodium falciparum proteomic data for 
      screening of potential vaccine candidates.
PG  - 136-43
LID - 10.1016/j.humimm.2009.11.009 [doi]
AB  - Advancement in the field of proteomics and bioinformatics offers tremendous 
      opportunities for the development of novel epitope-based effective vaccine 
      against human malaria. In this study, we have characterized 25 antigens as a 
      vaccine candidate and screened the potential T-lymphocyte epitopes presented by 
      human leukocyte antigen (HLA)-A, -B, and -DR molecules based on the proteomic 
      data of Plasmodium falciparum. Of the 25 proteins, 22 were predicted as probable 
      antigens and two were predicted as adhesions. In addition, seven proteins were 
      predicted to contain signal peptide for secretary pathway and six proteins were 
      found similar to the human and mouse reference proteins, whereas none of the 
      proteins were predicted as allergen. A total of 14,841 peptides were predicted as 
      epitope, presented by HLA class I and II supertypes that covered a broad human 
      population (approximately 95%). Our results suggest that HLA-based multistage and 
      multiepitope malaria vaccine would likely be needed to induce broader CD8(+) as 
      well as CD4(+) T-cell responses. The predicted epitopes may be served as a useful 
      diagnostic reagent for evaluating T-cell responses in the context of natural 
      infection and/or vaccine trials.
CI  - Copyright 2010 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Singh, Satarudra P
AU  - Singh SP
AD  - Amity Institute of Biotechnology, Amity University Uttar Pradesh, Lucknow, India.
FAU - Khan, Feroz
AU  - Khan F
FAU - Mishra, Bhartendu N
AU  - Mishra BN
LA  - eng
PT  - Journal Article
DEP - 20091231
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (Antigens, Protozoan)
RN  - 0 (Epitopes, T-Lymphocyte)
RN  - 0 (HLA Antigens)
RN  - 0 (Malaria Vaccines)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Antigens, Protozoan/*immunology
MH  - Epitope Mapping
MH  - Epitopes, T-Lymphocyte/*immunology
MH  - HLA Antigens/immunology
MH  - Humans
MH  - Malaria Vaccines/*immunology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Plasmodium falciparum/*immunology
MH  - Protein Structure, Tertiary
MH  - Proteomics
EDAT- 2009/12/01 06:00
MHDA- 2010/04/08 06:00
CRDT- 2009/12/01 06:00
PHST- 2009/06/27 00:00 [received]
PHST- 2009/10/09 00:00 [revised]
PHST- 2009/11/05 00:00 [accepted]
PHST- 2009/12/01 06:00 [entrez]
PHST- 2009/12/01 06:00 [pubmed]
PHST- 2010/04/08 06:00 [medline]
AID - S0198-8859(09)00640-5 [pii]
AID - 10.1016/j.humimm.2009.11.009 [doi]
PST - ppublish
SO  - Hum Immunol. 2010 Feb;71(2):136-43. doi: 10.1016/j.humimm.2009.11.009. Epub 2009 
      Dec 31.