PMID- 19945928
OWN - NLM
STAT- MEDLINE
DCOM- 20100413
LR  - 20100309
IS  - 1878-0539 (Electronic)
IS  - 1748-6815 (Linking)
VI  - 63
IP  - 4
DP  - 2010 Apr
TI  - Engineering cartilage substitute with a specific size and shape using porous 
      high-density polyethylene (HDPE) as internal support.
PG  - e370-5
LID - 10.1016/j.bjps.2009.10.016 [doi]
AB  - Despite the great advances in cartilage engineering, constructing cartilage of 
      large sizes and appropriate shapes remains a great challenge, owing to limits in 
      thickness of regenerated cartilage and to inferior mechanical properties of 
      scaffolds. This study introduces a pre-shaped polyglycolic acid (PGA)-coated 
      porous high-density polyethylene (HDPE) scaffold to overcome these challenges. 
      HDPE was carved into cylindrical rods and wrapped around by PGA fibres to form 
      PGA-HDPE scaffolds. Porcine chondrocytes were seeded into the scaffolds and the 
      constructs were cultured in vitro for 2 weeks before subcutaneous implantation 
      into nude mice. Scaffolds made purely of PGA with the same size and shape were 
      used as a control. After 8 weeks of implantation, the construct formed 
      cartilage-like tissue and retained its pre-designed shape and size. In addition, 
      the regenerated cartilage grew and completely surrounded the HDPE core, which 
      made the entire cartilage substitute biocompatible to its implanted environment 
      as native cartilage similarly does. By contrast, the shape and size of the 
      constructs in the control group seriously deformed and obvious hollow cavity and 
      necrotic tissue were observed in the inner region. These results demonstrate that 
      the use of HDPE as the internal support of a biodegradable scaffold has the 
      potential to circumvent the problems of limitations in size and shape, with 
      promising implications for the development of engineered cartilage appropriate 
      for clinical applications.
CI  - Copyright 2009 British Association of Plastic, Reconstructive and Aesthetic 
      Surgeons. Published by Elsevier Ltd. All rights reserved.
FAU - Wu, Yujia
AU  - Wu Y
AD  - Department of Plastic Surgery, Chang Zheng Hospital, The Second Military Medical 
      University, Shanghai, P.R. China.
FAU - Zhu, Lie
AU  - Zhu L
FAU - Jiang, Hua
AU  - Jiang H
FAU - Liu, Wei
AU  - Liu W
FAU - Liu, Yu
AU  - Liu Y
FAU - Cao, Yilin
AU  - Cao Y
FAU - Zhou, Guangdong
AU  - Zhou G
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091128
PL  - Netherlands
TA  - J Plast Reconstr Aesthet Surg
JT  - Journal of plastic, reconstructive & aesthetic surgery : JPRAS
JID - 101264239
RN  - 0 (Coated Materials, Biocompatible)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 9002-88-4 (Polyethylene)
SB  - IM
MH  - Animals
MH  - Animals, Newborn
MH  - Cartilage/*transplantation
MH  - Cells, Cultured
MH  - *Coated Materials, Biocompatible
MH  - Disease Models, Animal
MH  - Immunohistochemistry
MH  - Mice
MH  - Mice, Nude
MH  - *Polyethylene
MH  - *Polyglycolic Acid
MH  - Porosity
MH  - *Prostheses and Implants
MH  - Swine
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds
EDAT- 2009/12/01 06:00
MHDA- 2010/04/14 06:00
CRDT- 2009/12/01 06:00
PHST- 2009/06/01 00:00 [received]
PHST- 2009/10/02 00:00 [revised]
PHST- 2009/10/14 00:00 [accepted]
PHST- 2009/12/01 06:00 [entrez]
PHST- 2009/12/01 06:00 [pubmed]
PHST- 2010/04/14 06:00 [medline]
AID - S1748-6815(09)00741-4 [pii]
AID - 10.1016/j.bjps.2009.10.016 [doi]
PST - ppublish
SO  - J Plast Reconstr Aesthet Surg. 2010 Apr;63(4):e370-5. doi: 
      10.1016/j.bjps.2009.10.016. Epub 2009 Nov 28.