PMID- 19945964
OWN - NLM
STAT- MEDLINE
DCOM- 20100316
LR  - 20100114
IS  - 1460-2350 (Electronic)
IS  - 0268-1161 (Linking)
VI  - 25
IP  - 2
DP  - 2010 Feb
TI  - Influence of peritoneal fluid on the expression of angiogenic and proteolytic 
      factors in cultures of endometrial cells from women with endometriosis.
PG  - 398-405
LID - 10.1093/humrep/dep419 [doi]
AB  - BACKGROUND: Endometriosis, defined as the presence of endometrium outside the 
      uterus, is one of the most frequent benign gynaecological diseases. It has been 
      suggested that both endometrial and peritoneal factors, related to angiogenesis 
      and proteolysis, can be implicated in this disease. The aim of this study was to 
      evaluate the influence of peritoneal fluid on the expression of angiogenic and 
      proteolytic factors in cultures of endometrial cells from women with and without 
      endometriosis. METHODS: Endometrial cells were isolated, cultured and treated 
      with endometriotic or normal peritoneal fluid. Vascular endothelial growth 
      factor-A (VEGF-A), urokinase plasminogen activator (uPA), matrix 
      metalloproteinase-3 (MMP-3) and their inhibitors including thrombospondin-1, 
      plasminogen activator inhibitor-1 and MMP inhibitor type 1 (TIMP-1) mRNA levels 
      were evaluated by quantitative RT-PCR, and protein levels were quantified by 
      ELISA. RESULTS: Peritoneal fluid from women with endometriosis induced an 
      increase in VEGF-A and uPA protein and VEGF-A mRNA and uPA mRNA levels in 
      endometrial cell culture from women with (P < 0.01) and without endometriosis (P 
      < 0.05). The highest levels of VEGF-A and uPA were observed in endometrial cell 
      cultures from patients with endometriosis and treated with peritoneal fluid from 
      women with endometriosis. CONCLUSIONS: Peritoneal fluid from women with 
      endometriosis induced more VEGF and uPA expression in endometrial cell culture 
      from women with endometriosis than did normal peritoneal fluid. 
      Endometrial-peritoneal interactions increased angiogenic and proteolytic factors 
      in endometrial cells, which could contribute to the development of endometriotic 
      lesions.
FAU - Cosin, R
AU  - Cosin R
AD  - Research Center, Hospital Universitario La Fe, Valencia, Spain.
FAU - Gilabert-Estelles, J
AU  - Gilabert-Estelles J
FAU - Ramon, L A
AU  - Ramon LA
FAU - Gomez-Lechon, M J
AU  - Gomez-Lechon MJ
FAU - Gilabert, J
AU  - Gilabert J
FAU - Chirivella, M
AU  - Chirivella M
FAU - Braza-Boils, A
AU  - Braza-Boils A
FAU - Espana, F
AU  - Espana F
FAU - Estelles, A
AU  - Estelles A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20091126
PL  - England
TA  - Hum Reprod
JT  - Human reproduction (Oxford, England)
JID - 8701199
RN  - 0 (Angiogenic Proteins)
RN  - 0 (Plasminogen Activator Inhibitor 1)
RN  - 0 (Thrombospondin 1)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 0 (VEGFA protein, human)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 3.4.- (Peptide Hydrolases)
RN  - EC 3.4.21.73 (Urokinase-Type Plasminogen Activator)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adult
MH  - Angiogenic Proteins/*biosynthesis
MH  - Ascitic Fluid/*physiology
MH  - Cells, Cultured
MH  - Endometriosis/*metabolism
MH  - Endometrium/*metabolism
MH  - Female
MH  - Humans
MH  - Matrix Metalloproteinase 3/biosynthesis
MH  - Peptide Hydrolases/*biosynthesis
MH  - Plasminogen Activator Inhibitor 1/biosynthesis
MH  - Thrombospondin 1/biosynthesis
MH  - Tissue Inhibitor of Metalloproteinase-1/biosynthesis
MH  - Urokinase-Type Plasminogen Activator/biosynthesis
MH  - Vascular Endothelial Growth Factor A/biosynthesis
EDAT- 2009/12/01 06:00
MHDA- 2010/03/17 06:00
CRDT- 2009/12/01 06:00
PHST- 2009/12/01 06:00 [entrez]
PHST- 2009/12/01 06:00 [pubmed]
PHST- 2010/03/17 06:00 [medline]
AID - dep419 [pii]
AID - 10.1093/humrep/dep419 [doi]
PST - ppublish
SO  - Hum Reprod. 2010 Feb;25(2):398-405. doi: 10.1093/humrep/dep419. Epub 2009 Nov 26.