PMID- 19946349
OWN - NLM
STAT- MEDLINE
DCOM- 20100527
LR  - 20181201
IS  - 1028-852X (Print)
IS  - 1028-852X (Linking)
VI  - 13
IP  - 4
DP  - 2009 Oct
TI  - Apoptotic and necrotic effects of pectic acid on rat pituitary GH3/B6 tumor 
      cells.
PG  - 229-36
AB  - BACKGROUND: Pectin is composed of complex polysaccharides that can inhibit cancer 
      metastasis and proliferation with no evidence of toxicity. In the present study, 
      the apoptotic and necrotic effects of pectic acid (PA) on the rat pituitary 
      GH3/B6 tumor cells has been investigated. METHODS: GH3/B6 cells were cultured in 
      the Ham's F12 medium enriched with 15% horse serum and 2.5% fetal bovine serum 
      for 3 days. Then, they were treated by various amounts of PA in different periods 
      (6, 24 and 48 hours). Bromocriptine was used as positive control and the cell 
      viability was detected by MTT test. The nuclear morphology of cells was explored 
      by florescent stains including acridine orange (AO)/ethidium bromide (EB). In 
      addition, percentages of apoptotic and necrotic cells were studied with 
      triphosphate nick-end labeling (TUNEL) assay, cell cycle analysis and propidium 
      iodide (PI) staining. RESULTS: Long-term incubation with PA results in increased 
      cell death and DNA damage as detected by MTT assay and AO/EB staining. TUNEL 
      assay showed that PA (100 microg/ml to 1 mg/ml) could induce apoptosis in a 
      dose-dependent manner, while higher concentrations of PA (2.5 and 5 mg/ml) 
      induced necrosis which was confirmed by PI staining. Furthermore, cell cycle 
      analysis indicated that PA induced sub G1 events, and DNA fragmentation was also 
      correlated with the number of the apoptotic cells. CONCLUSION: It can be 
      concluded that PA is responsible for apoptosis in the rat pituitary tumor cells. 
      Therefore, one may suggest that this group of polysaccharides can be used in 
      treatment of pituitary tumors.
FAU - Attari, Farnoosh
AU  - Attari F
AD  - Dept. of Animal Biology, School of Biology, College of Scienc, University of 
      Tehran, Tehran, Iran.
FAU - Sepehri, Houri
AU  - Sepehri H
AD  - Dept. of Animal Biology, School of Biology, College of Scienc, University of 
      Tehran, Tehran, Iran.
FAU - Delphi, Ladan
AU  - Delphi L
AD  - Dept. of Animal Biology, School of Biology, College of Scienc, University of 
      Tehran, Tehran, Iran.
FAU - Goliaei, Bahram
AU  - Goliaei B
AD  - Institute of Biochemistry and Biophysics (IBB), University of Tehran, Tehran, 
      Iran.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Iran
TA  - Iran Biomed J
JT  - Iranian biomedical journal
JID - 9814853
RN  - 0 (Nucleosomes)
RN  - 89NA02M4RX (Pectins)
RN  - VV3XD4CL04 (polygalacturonic acid)
SB  - IM
MH  - Animals
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Membrane Permeability
MH  - Cell Survival/drug effects
MH  - DNA Fragmentation/drug effects
MH  - In Situ Nick-End Labeling
MH  - Microscopy, Fluorescence
MH  - Necrosis
MH  - Nucleosomes/pathology
MH  - Pectins/*pharmacology
MH  - Pituitary Neoplasms/*drug therapy/*pathology
MH  - Rats
OTO - NOTNLM
OT  - Pectic Acid
OT  - Apoptosis
OT  - Necrosis
OT  - GH3/B6 cells
EDAT- 2009/12/01 06:00
MHDA- 2010/05/28 06:00
CRDT- 2009/12/01 06:00
PHST- 2009/12/01 06:00 [entrez]
PHST- 2009/12/01 06:00 [pubmed]
PHST- 2010/05/28 06:00 [medline]
PST - ppublish
SO  - Iran Biomed J. 2009 Oct;13(4):229-36.